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In situ dissection of domain boundaries affects genome topology and gene transcription in Drosophila

ABSTRACT: In situ dissection of domain boundaries affects genome topology and gene transcription in Drosophila

PROVIDER: PRJNA561360 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10009462_1.fastq.gz	Fastqsanger.gz
	SRR10009462_2.fastq.gz	Fastqsanger.gz
	SRR10009463_1.fastq.gz	Fastqsanger.gz
	SRR10009463_2.fastq.gz	Fastqsanger.gz
	SRR10009464_1.fastq.gz	Fastqsanger.gz

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In situ dissection of domain boundaries affects genome topology and gene transcription in Drosophila [Hi-C]

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In situ dissection of domain boundaries affects genome topology and gene transcription in Drosophila [RNA-seq]

Project description:The molecular mechanisms responsible for Topologically Associated Domains (TADs) formation are not yet fully understood. In Drosophila, it has been proposed that transcription is fundamental for TAD organization while the participation of genetic sequences bound by Architectural Proteins (APs) remains controversial. Here, we investigate the contribution of domain boundaries to TAD organization and the regulation of gene expression at the Notch gene locus in Drosophila. We find that deletion of domain boundaries results in TAD fusion and long-range topological defects that are accompanied by loss of APs and RNA Pol II chromatin binding as well as in defects in transcription. Together, our results provide compelling evidence on the contribution of discrete genetic sequences bound by APs and RNA Pol II in the partition of the genome into TADs and in the regulation of gene expression in Drosophila

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In situ dissection of domain boundaries affects genome topology and gene transcription in Drosophila

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In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila.

Project description:Chromosomes are organized into high-frequency chromatin interaction domains called topologically associating domains (TADs), which are separated from each other by domain boundaries. The molecular mechanisms responsible for TAD formation are not yet fully understood. In Drosophila, it has been proposed that transcription is fundamental for TAD organization while the participation of genetic sequences bound by architectural proteins (APs) remains controversial. Here, we investigate the contribution of domain boundaries to TAD organization and the regulation of gene expression at the Notch gene locus in Drosophila. We find that deletion of domain boundaries results in TAD fusion and long-range topological defects that are accompanied by loss of APs and RNA Pol II chromatin binding as well as defects in transcription. Together, our results provide compelling evidence of the contribution of discrete genetic sequences bound by APs and RNA Pol II in the partition of the genome into TADs and in the regulation of gene expression in Drosophila.

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