Project description:To find out the potential targets in response to estrogen treatment on ACHN cell, a human renal cell carcinoma (RCC) cell line. Since estrogen can repress ACHN growth in a partly estrogen receptor-dependent manner, it is possible that phosphorylation state in ACHN cells is regulated.
Project description:DNase-seq on cell line ACHN (epithelial cell from 22 year old male human kidney, renal cell adenocarcinoma) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:Kindlin-2, an integrin-interacting FERM-domain-containing protein, has been known to play critical roles for tumor progression. However, the role of Kindlin-2 in renal cell carcinoma (RCC) progression has not been reported. We aim to investigate the role of Kindlin-2 in the progression of RCC and the underlying mechanisms. To uncover the related pathway in which Kindlin-2 is involved to promote clear cell renal cell carcinoma progression, ACHN control and Kindlin-2-depleting cells were analyzed by Affymetrix GeneChip human Gene 1.0 ST Arrays. ACHN cells were transfected with control short hairpin RNA (shRNA) or Kindlin-2 shRNA. ACHN control and Kindlin-2-depleting cells cDNAs were hybridized to Affymetrix GeneChip Human Gene 1.0 ST arrays. Data were analyzed by Expression Console 1.4.1.
Project description:Piericidin A (PA), the most common member of the family of piericidins, is fermented from Streptomyces#sp.HBERC#58855. We have found that PA had significant cytostatic activity against the ACHN cells, which can be used as a potential drug against renal cell carcinoma (RCC). Therefore, we used the second-generation transcriptome sequencing (RNA-seq) to determine the expression changes of various genes in ACHN cells treated with PA (25nM,50nM) or DMSO (0.1%), so that we can investigate the molecular mechanism of PA against the RCC.
Project description:Foam cells are dysfunctional, lipid-laden macrophages associated with chronic inflammation of infectious and non-infectious origin. To test the hypothesis that foam cell biogenesis is disease-specific, we compared bulk transcriptomics data obtained by RNA seq in human monocyte derived macrophages (MDM) subjected to two types of infections in vitro. One was with Mycobacterium tuberculosis and the other with Cryptococcus neoformans. We also exposed MDM to cell-free conditioned medium from cultures of the ACHN cell line, which is derived from a human renal cell carcinoma, to study foam cell formation in the context of papillary renal cell carcinoma (pRCC). We found that, with both infections, the accumulation of TAG results from decreased oxidative phosphorylation, increased glycolysis, increased lipid biosynthesis, and decreased lipid catabolism. However, the molecular modalities of
Project description:Renal cell carcinoma (RCC) is the most common histological subtype of kidney cancer. Although targeted therapies and immune checkpoint inhibitors have markedly improved patient outcomes, their efficacy remains limited. In recent years, photodynamic therapy (PDT) has drawn increasing attention for its role in cancer treatment. To investigate the effects of the novel porphyrin-based photosensitizer meso-5-[ρ-DTPA-aminophenyl]-10,15,20 triphenylporphyrin (DTP) on RCC, we employed the human renal tubular epithelial cell line HK-2, human RCC cell lines 786-O, ACHN, and A498, as well as the murine renal carcinoma cell line Renca. We assessed intracellular DTP uptake, phototoxicity, and subcellular localization, and further explored potential mechanisms through next-generation sequencing. Our results showed that DTP uptake was significantly higher in RCC cells than in normal renal tubular epithelial cells, and DTP-PDT exerted pronounced phototoxic effects on RCC cells. Through sequencing and in vitro analyses, we found that DTP-PDT markedly reduced cholesterol levels in RCC cells while inducing compensatory upregulation of HMGCR. In vivo experiments further demonstrated that combining DTP-PDT with statins produced stronger anticancer effects. Overall, this study provides compelling evidence that DTP-PDT exhibits potent cytotoxic activity against renal cell carcinoma, and that statins can further enhance its therapeutic efficacy.
Project description:Kindlin-2, an integrin-interacting FERM-domain-containing protein, has been known to play critical roles for tumor progression. However, the role of Kindlin-2 in renal cell carcinoma (RCC) progression has not been reported. We aim to investigate the role of Kindlin-2 in the progression of RCC and the underlying mechanisms. To uncover the related pathway in which Kindlin-2 is involved to promote clear cell renal cell carcinoma progression, ACHN control and Kindlin-2-depleting cells were analyzed by Affymetrix GeneChip human Gene 1.0 ST Arrays.
Project description:Among all types of urological cancers, the clear cell renal cell carcinoma is the second leading cause of death in adults. This is mainly due to lack of promising prognosis or predictors, and effective target therapy. S100A6 (calcyclin), a member of S100 family of proteins, is reported to be elevated in many types of cancers. In the present study, we analyzed the expression of S100A6 in mRNA, in proteins and tissues. The mechanism of enhancing tumorigenesis was studied to understand the role of S100A6 in clear cell renal cell carcinoma tumorigenesis. Microarray and bioinformatic analyses were performed in the stable transfection of overexpression and knockdown of S100A6, comparing with each empty vector control in order to find the different expression genes and explore the mechanism. The microarray analysis of clear cell renal cell carcinoma 786-O cell line treated with overexpression and knockdown S100A6. Four samples: overexpression S100A6 sample and vector control sample, knockdown S100A6 and vector control sample, each sample had three replicates.