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Analysis of Drosophila melanogaster Chromosome Topology in Larval Eye and Antennal Discs

ABSTRACT: Analysis of Drosophila melanogaster Chromosome Topology in Larval Eye and Antennal Discs

PROVIDER: PRJNA561788 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10027500_1.fastq.gz	Fastqsanger.gz
	SRR10027500_2.fastq.gz	Fastqsanger.gz
	SRR10027501_1.fastq.gz	Fastqsanger.gz
	SRR10027501_2.fastq.gz	Fastqsanger.gz
	SRR10027502_1.fastq.gz	Fastqsanger.gz

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Analysis of Drosophila melanogaster Chromosome Topology in Larval Eye and Antennal Discs

Project description:The goal of this study was to examine chromosome topology in Drosophila larval eye and antennal discs by identifying topologically associating domains (TADs) across the genome. TADs were compared between the eye and antennal disc to determine whether they contribute to cell-type-specific homologous pairing and transvection.

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Project description:In this study we use Tag-sequencing in eye-antennal and wing imaginal discs across Drosophila species to determine a set of conserved eye-specific developmental genes. Next, we perform motif discovery analysis using the tool i-cisTarget, to depict the core gene developmental network underlying compound eye photoreceptor. The Glass position weight matrix appears as the most highly overrepresented motif, thus positioning Glass as a master regulator in compound eye photoreceptor development. Differential gene expression analysis by RNA-seq in D.melanogaster wild-type eye-antennal versus glass mutant [gl 60j] shows that the majority of our predicted Glass targets show strong downregulation in the glass mutant. This SuperSeries is composed of the following subset Series: GSE39781: RNA-seq in wild-type and glass mutant eye-antennal discs in Drosophila melanogaster GSE39782: Tag-seq profiling in eye-antennal and wing imaginal discs of D. melanogaster, D. yakuba and D. virilis

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