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West Nile virus infection of human monocyte-derived dendritic cells

ABSTRACT: West Nile virus infection of human monocyte-derived dendritic cells

PROVIDER: PRJNA562253 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10044800.fastq.gz	Fastqsanger.gz
	SRR10044801.fastq.gz	Fastqsanger.gz
	SRR10044802.fastq.gz	Fastqsanger.gz
	SRR10044803.fastq.gz	Fastqsanger.gz
	SRR10044704.fastq.gz	Fastqsanger.gz

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West Nile virus infection of human monocyte-derived dendritic cells

Project description:West Nile virus (WNV) is a neurotropic flavivirus and the leading cause of mosquito-borne encephalitis in the United States. Recent studies in humans have found that dysfunctional T cell responses strongly correlate with development of severe WNV neuroinvasive disease. However, the contributions of human dendritic cells (DCs) in priming WNV-specific T cell immunity remains poorly understood. Here, we demonstrate that human monocyte derived DCs (moDCs) support productive viral replication following infection with a pathogenic strain of WNV. Antiviral effector gene transcription was strongly induced during the log-phase viral growth, while secretion of type I interferons (IFN) occurred with delayed kinetics. Activation of RIG-I like receptor (RLR) or type I IFN signaling prior to log-phase viral growth significantly diminished viral replication, suggesting that early activation of antiviral programs can block WNV infection. In contrast to the induction of antiviral responses, WNV infection did not promote transcription or secretion of pro-inflammatory (IL-6, GM-CSF, CCL3, CCL5, CXCL9) or T cell modulatory cytokines (IL-4, IL-12, IL-15). There was also minimal induction of molecules associated with antigen presentation and T cell priming, including the co-stimulatory molecules CD80, CD86, and CD40. Functionally, WNV-infected moDCs dampened allogenic CD4 and CD8 T cell activation and proliferation. Combined, we propose a model where WNV subverts human DC activation to compromise priming of WNV-specific T cell immunity.

2019-09-06 | GSE136342 | GEO

Mouse primary myeloid dendritic cell transcriptome response to a wild type infectious clone of West Nile virus (WNVMT) and mutant virus (WNVE218A).

Project description:The purpose is to obtain samples for mRNA analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A).

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Primary mouse myeloid dendritic cell transcriptome response to a wild type infectious clone of West Nile virus (WNVMT) and mutant virus (WNVE218A).

Project description:The purpose is to obtain samples for mRNA analysis in primary mouse myeloid dendritic cells infected with wild type West Nile virus (WNVMT) and mutant virus (WNVE218A).

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Project description:High throughput sequencing was performed using Illumina HiSeq to identify differentially regulated genes in Culex mosquitoes after West Nile virus infection.

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Project description:Proteomics data from 2x105 dendritic cells; West Nile Virus treated, Untreated, Mockulum treated; Time points 0, 4, 8, 12, 24; Two biological replicates

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Mouse dendritic cell metabolome response to wild-type West Nile virus (WNV-NY)

Project description:Metabolomic data from 2x105 dendritic cells; West Nile Virus treated, Untreated, Mockulum treated; Time points 0, 4, 8, 12, 24; Two biological replicates

2015-06-11 | MSV000079149 | MassIVE

Profiling the successful response to infection with West Nile virus

Project description:Individual variations in immune status and function determine responses to infection and contribute to disease severity and outcome. Patients exhibit considerable variation in clinical responses to infection with West Nile virus. We have undertaken a comprehensive characterization of the immune responses of a stratified cohort of patients with a history of West Nile virus infection to identify key mechanisms of resistance and susceptibility. We provide molecular profiles of cellular mechanisms of primary human immune cells defined through multifaceted interrogation including multiplexed gene expression analysis integrated with highly sensitive multidimensional flow cytometry. The availability of reliably curated patient cohorts and data-sharing and data mining techniques of high-throughout investigations should accelerate identification of critical elements of immune resistance to important pathogens

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A Mouse Model of Chronic West Nile Virus Infection

Project description:Here, we characterize the RIX line CC(032x013)F1, which serves as a mouse model of chronic WNV infection. While studies using C57BL/6 mice have shown that WNV RNA can persist in the CNS up to 3 months post infection in a limited fraction of mice (Appler et al., 2010), to date there is a lack of a robust mouse model of chronic West Nile virus infection that can be used to elucidate the immune responses associated with this viral persistence and chronicity of symptoms described in human patients. Here, we characterize this line in comparison with lines showing either no disease symptoms or significant disease, and suggest a mechanism by which WNV infection can become chronic through alterations in immune responses. Microarrays were performed on spleen samples from mice collected at days 7,12,21,28 post-infection with west nile virus or from time-matched mock-infected animals.

2016-06-01 | E-GEOD-82046 | biostudies-arrayexpress

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