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Soil metagenome

ABSTRACT: Artificial Root Exudates Incubation experiment

PROVIDER: PRJNA562427 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10029900_1.fastq.gz	Fastqsanger.gz
	SRR10029900_2.fastq.gz	Fastqsanger.gz
	SRR10029901_1.fastq.gz	Fastqsanger.gz
	SRR10029901_2.fastq.gz	Fastqsanger.gz
	SRR10029902_1.fastq.gz	Fastqsanger.gz

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Project description:Purpose: The recent publication of the fungal mutualist R. irregularis genome facilitated transcriptomic studies. We here adress the gene regulation of R. irregularis in response to root exudates from rice wild-type and osnope1 (no perception candidate - mutant unable to host arbuscular mycorrhizal fungi) Methods: Spores of R. irregularis were treated with root exudates and collected at 1 hour, 24 hours and 7 days after addition. To monitor fungal gene regulation, control conditions were also prepared at T0, 1h, 24h and 7d. mRNA were sequenced by HiSeq Illumina. Reads were mapped on the Rhizophagus irregularis genome assembly (Gloin1 - Tisserant et al., PNAS, 2013) using CLCworkbench suite. Results: -At 1h, a set of 92 fungal genes were found up-regulated in response to wt root exudates (92), not to osnope1 root exudates, many of them being involved in cell signaling. -At 24h and 7d, numerous genes putatively involved in primary metabolism were up-regulated in response to wt root exudates, not in response to osnope1 root exudates -Several vital genes involved in cell development are repressed in response to osnope1 RE compared to wt RE. Conclusions: these results argue for a high metabolic activity induced by wt root exudates, not by osnope1 root exudates.

2017-02-04 | GSE65595 | GEO

The responses of CHA0 treated with WT(Col-0) root exudates, Gln and sgn3 myb36 root exudates

Project description:In our previous work, we found that the root exudates of sgn3 myb36 promoted the colonization of CHA0 on roots. Through LC-MS, we identified a large amount of glutamine (Gln) in the root exudates of sgn3 myb36. Therefore, we aim to use RNA-seq to uncover whether the root exudates of sgn3 myb36 and Gln have the same regulatory effects on CHA0. By conducting differential analysis with the CK (CHA0 treated with wild-type root exudates), we hope to identify the specific regulatory mechanisms of sgn3 myb36 and Gln on CHA0.

2024-11-06 | GSE280681 | GEO

Transcriptomics of Bacillus amyloliquefaciens SQR9 to maize root exudates

Project description:Root exudates play an important role in plant-microbe interaction. The transcriptional profilings of plant growth-promoting rhizobacteria Bacillus amyloliquefaciens SQR9 in response to maize root exudates under static condition, were investigated by an Illumina RNA-seq for understanding the regulatory roles of the root exudates. 4 treatments, including 2 blank control (24 h and 48 h-post inoculation, named as 5 and 15, respectively), and 2 treatments with maize root exudates (24 h and 48 h-post inoculation, named as 7 and 17, respectively)

2014-11-01 | E-GEOD-54987 | biostudies-arrayexpress

Borriss_FZB42_IE-RE

Project description:Transcription profiling by array of Bacillus amyloliquefaciens strain FZB42 after interaction exudates (IE) treatment, at OD600=1.0 and at OD600=1.0 respectively. IE was the root exudates prepared from maize plants growing with FZB42. The reference was treated with the root exudates (RE), prepared from maize plants grown in an axenic system.

2012-04-15 | E-MEXP-3553 | biostudies-arrayexpress

Differential gene expression of endosphere and soil Bacillus mycoides isolate in response to root exudates using RNA sequencing

Project description:In general, the endosphere isolate EC18 showed more numbers of genes significantly altered in the presence of root exudates than the soil isolate SB8 . Some of the altered genes in the two strains showed overlap. Some of these genes were previously reported to be involved in microbe-plant interactions, such as organic substance metabolism, oxidation reduction, transmembrane transportation and a subset with putative or unknown function. It was also found some genes showed opposite trend among the two strains.

2017-04-26 | GSE98148 | GEO

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