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Dynamic study of the cell wall integrity signaling pathway in Aspergillus nidulans

ABSTRACT: Dynamic study of the cell wall integrity signaling pathway in Aspergillus nidulans

PROVIDER: PRJNA562694 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10042300.fastq.gz	Fastqsanger.gz
	SRR10042301.fastq.gz	Fastqsanger.gz
	SRR10042302.fastq.gz	Fastqsanger.gz
	SRR10042303.fastq.gz	Fastqsanger.gz
	SRR10042304.fastq.gz	Fastqsanger.gz

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Dynamic study of the cell wall integrity signaling pathway in Aspergillus nidulans

Project description:A phosphoproteomic evaluation of the cellular response to micafungin (cell wall perturbant) in the filamentous fungi, A. nidulans, reveals putative, currently unknown cell wall integrity signaling (CWIS) associated proteins. This study used thirteen timepoints were taken from 0 to 10 minutes after cell wall exposure with two biological and two technical replicates. All data was processed using label free quantification with MaxQuant software.

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Aspergillus nidulans dynamic transcriptome during synchronized conidiation

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Dynamic study of the cell wall integrity signaling pathway in Aspergillus nidulans

Project description:This study aims to determine the downstream impact of cell wall perturbation on the filamentous fungus, Aspergillus nidulans.Fungi were treated with micafungin during mid-exponential growth phase and RNA-sequencing was performed at 0, 5, 10, 15, 20, 25, 30, 40, 50, 60, 75, 90, and 120 minutes of exposure in 3 biological replicates.

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RNA-seq analysis of kinase deletion mutant (ΔmpkA) and parental strain Aspergillus nidulans reveal novel cellular functions of MpkA.

Project description:A transcriptomic assessment of a kinase deletion mutant (ΔmpkA) and a control strain revealed many cellular activities this kinase is impacting. Studies were conducted in the model filamentous fungi, Aspergillus nidulans¸ under rich growth conditions. MpkA is a known kinase involved in the cell wall integrity signaling pathway. This pathway is activated upon cell wall stress and regulates production of cell wall related genes. Here, fungi were grown under non-stressful conditions. The comparison between the control strain and the deletion mutant transcript levels revealed previously unknown functions, such as iron starvation, that MpkA may be involved with.

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A seven-membered cell wall related transglycosylase gene family in Aspergillus niger is relevant for cell wall integrity in cell wall mutants with reduced α-glucan or galactomannan

Project description:Chitin is an important fungal cell wall component that is cross-linked to β-glucan for structural integrity. Acquisition of chitin to glucan cross-links has previously been shown to be performed by transglycosylation enzymes in S. cerevisiae, called Congo Red hypersensitive (Crh) enzymes. Here, we characterized the impact of deleting all seven gene members of the crh gene family (crhA-G) in Aspergillus niger on cell wall integrity, cell wall composition and genome-wide gene expression. In this study, we show that the seven-fold knockout strain shows slightly compact plate growth, but no increased sensitivity to cell wall perturbing compounds. Additionally, we found that the cell wall composition of this knockout strain was virtually identical to that of the wild type. In congruence with these data, genome-wide expression analysis revealed very limited changes in gene expression and no signs of activation of the cell wall integrity response pathway. However, deleting the entire crh gene family in cell wall mutants that are deficient in either galactofuranose (ΔugmA) or α-glucan (ΔagsE and ΔagsA, ΔagsE), resulted in a synthetic growth defect and an increased sensitivity towards Congo Red compared the parental strains. Altogether, these results indicate that loss of the crh gene family in A. niger does not trigger the cell wall integrity response in itself, but does play an important role in ensuring cell wall integrity in mutant strains with reduced galactofuranose or α-glucan.

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Aspergillus nidulans dynamic transcriptome during synchronized conidiation

Project description:Aspergillus nidulans dynamic transcriptome during synchronized conidiation

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Project description:Functional analysis of Mpk1-mediated cell wall integrity signaling pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha

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Project description:Cryptococcus neoformans is a fungal pathogen of immunocompromised people that causes fatal meningitis. The fungal cell wall is essential to viability and pathogenesis of C. neoformans, and biosynthesis and repair of the wall is primarily controlled by the cell wall integrity (CWI) signaling pathway. Previous work by us and others have shown that deletion of genes encoding the four major kinases in the CWI signaling pathway, namely PKC1, BCK1, MKK2 and MPK1 results in severe cell wall phenotypes, sensitivity to a variety of cell wall stressors and, for Mpk1, reduced virulence in a mouse model. Here, we examined the global transcriptional response of gene deletions of BCK1, MKK2 and MPK1 compared to wild-type cells. We found over 1000 genes were differentially expressed in one or more of the deletion strains, with 115 genes differentially expressed in all three strains, many of which have been identified as genes regulated by the cAMP/protein kinase A (PKA) pathway. Biochemical measurements of cAMP levels in the kinase deletion stains revealed significantly less cAMP in all of the deletion strains compared to wild-type cells. The deletion strains also produced significantly smaller capsules than wild type KN99 under capsule inducing conditions, although they shed similar levels of capsule as wild type. Finally, addition of exogenous cAMP led to reduced sensitivity to cell wall stress and restored surface capsule to near wild-type levels. Thus, we have direct evidence of cross talk between the CWI and cAMP/PKA pathways that may have important implications for regulation of cell wall and capsule homeostasis. Comparison of three gene deletion strains to wild-type, with 3 biological replicates for each sample.

2014-07-01 | E-GEOD-57217 | biostudies-arrayexpress

Functional analysis of Mpk1-mediated cell wall integrity signaling pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha

Project description:Purpose: To identify the downstream gene sets regulated by Mpk1p in H. polymorpha under normal and caffeine-treated conditions, we compared the RNA-seq based transcriptional profiles. Methods: Total RNA was isolated according to the hot phenol extraction method and all procedure for RNA sequencing were conducted by ChunLab. More than 1.5-fold regulated genes are functionally classified using the Munich Information Center for Protein Sequences (MIPS) FunCat. Results: Transcriptome analysis of the DL1-L strain revealed that caffeine significantly increased expression of a subset of cell-wall related genes in an Mpk1p-dependent manner, but most of which were not Rlm1-target genes. Conclusion: Our results support the essential role of Mpk1p in maintaining cell wall integrity upon several stress conditions of H. polymorpha.

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Transcriptome analysis of isoxaben-induced loss of cell wall integrity in Arabidopsis nia1/2 mutant

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2018-01-25 | GSE109613 | GEO

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