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Transcriptome profiling of tdc and agdi clusters deletion mutant of Enterococcus faecalis V583.

ABSTRACT: Transcriptome profiling of tdc and agdi clusters deletion mutant of Enterococcus faecalis V583.

PROVIDER: PRJNA564108 | ENA |

REPOSITORIES: ENA

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Transcriptome profiling of tdc and agdi clusters deletion mutant of Enterococcus faecalis V583.

Project description:The aim was to study the transcriptional profiling of the tdc and agdi clusters delection mutant E. faecalis V583 ΔtdcΔagdi (non-tyramine non-putrescine producer) compared to the wild type strain E. faecalis V583 (tyramine producer). We compared the expression profile of the strains grown in M17 medium with glucose as carbon source and suplemented with tyrosine.

2019-09-06 | GSE136953 | GEO

Transcriptome profiling of tdc cluster delection mutant of Enterococcus faecalis V583

Project description:The aim was to study the transcriptional profiling of the tdc cluster delection mutant E. faecalis V583 Î?tdc (non-tyramine producer) compared to the wild type strain E. faecalis V583 (tyramine producer). We compared the expression profile of the strains grown in M17 medium with glucose as carbon source and suplemented with tyrosine. E. faecalis V583 Î?tdc cells (test) compared with E. faecalis V583 cells (reference). Both strains grown in GM17 medium suplemented 15 mM tyrosine.

2016-02-15 | E-GEOD-77864 | biostudies-arrayexpress

Transcriptome profiling of tdc cluster delection mutant of Enterococcus faecalis V583

Project description:The aim was to study the transcriptional profiling of the tdc cluster delection mutant E. faecalis V583 Δtdc (non-tyramine producer) compared to the wild type strain E. faecalis V583 (tyramine producer). We compared the expression profile of the strains grown in M17 medium with glucose as carbon source and suplemented with tyrosine.

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The Enterococcus faecalis V583 transcriptional profile during survival within macrophages

Project description:Transcriptional profiling of E. faecalis V583 during intracellular survival compared to V583 grown to mid-log phase (OD 600 = 0.05) in THB + 1% glucose. In this study we report the complete intracellular E. faecalis V583 transcriptome following infection of RAW264.7 macrophages. During intracellular survival, approximately 45% of the V583 genome was differentially regulated including numerous genes involved in the oxidative stress, heat shock and SOS responses. We observed that the E. faecalis-containing phagosome was limited for glycolytic substrates, nucleotides, amino acids and numerous ions necessary for growth and protein function. Approximately 35% of the genes differentially regulated during survival within macrophages were of hypothetical/unknown function, suggesting that the V583 response to phagocytosis involves many previously unstudied loci. Here, we provide the first comprehensive study elucidating the transcriptional response of E. faecalis to phagocytosis, which may provide new targets for future studies.

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Enterococcus Faecalis V583 secretome

Project description:Identification of proteins in the secretome of Enterococcus Faecalis V583 after growth on urine

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The Enterococcus faecalis V583 transcriptional profile during survival within macrophages

2011-12-21 | E-GEOD-34590 | biostudies-arrayexpress

Transcriptome profiling of TDC cluster deletion mutant of Enterococcus faecalis V583.

Project description:The species Enterococcus faecalis is able to catabolise the amino acid tyrosine into the biogenic amine tyramine by the tyrosine decarboxilase (TDC) pathway Ladero et al. (2012) [1]. The TDC cluster comprises four genes: tyrS, an aminoacyl-tRNA synthetase-like gene; tdcA, which encodes the tyrosine decarboxylase; tyrP, a tyrosine/tyramine exchanger gene and nhaC-2, which encodes an Na(+)/H(+) antiporter and whose role in the tyramine biosynthesis remains unknown [2]. In E. faecalis V583 the last three genes are co-transcribed as a single polycistronic mRNA forming the catabolic operon, while tyrS is transcribed independently of the catabolic genes as a monocistronic mRNA [2]. The catabolic operon is transcriptionally induced by tyrosine and acidic pH. On the opposite, the tyrS expression is repressed by tyrosine concentrations [2]. In this work we report the transcriptional profiling of the TDC cluster deletion mutant (E. faecalis V583 ΔTDC) [2] compared to the wild-type strain, both grown in M17 medium supplemented with tyrosine. The transcriptional profile data of TDC cluster-regulated genes were deposited in the Gene Expression Omnibus (GEO) database under accession no. GSE77864.

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Comparative genomic hybridization of several Enterococcus faecalis strains compared to strain V583

Project description:Gene content in various Enterococcus faecalis strains compared to E. faecalis V583. Strains have been compared to the V583 strain by comparative genomic hybridization using genome-wide PCR-based microarrays representing the V583 genome. Genes have been deemed "present" or "divergent" in the various strains.

2007-01-15 | E-MEXP-931 | biostudies-arrayexpress

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