Project description:H1703, H1299, and H358T cells treated with or without si-ETV4 were used to screen the global profiling of human long-noncoding RNAs (lncRNAs) and protein-coding transcripts.
Project description:The ETS family of oncogenic TFs is emerging as crucial mediators of tumorigenesis in solid tumors. New insights into the molecular mechanisms hijacked by these factors will pave the way for novel therapeutic strategies. We have previously shown that ETV4 is the preponderant ETS factor that is associated with tumor progression and a worse prognosis in NSCLC. To determine the genome-wide chromatin binding of ETV4, we performed chromatin immunoprecipitation (ChIP)-sequencing studies in A549-shETV4 cells transfected with flag-ETV4 using flag antibody. In total, 54389 ETV4 binding peaks representing 25708 genes were identified (FC > 2), and 43.71% peaks were enriched in the promoter regions. Our ChIP-seq data showed that ETV4 is enriched in many genes that related with the wnt signaling pathway, MAPK signaling pathway, cell cycle, and autophagy, etc.
Project description:Our in vitro binding studies support a model whereby MED25 exhibits multivalent interactions with a subset of related ETS factors, ETV1/4/5. We hypothesize that the interaction would allow for coregulation of genes by ETV1/4/5 and MED25, acting perhaps to link the ETVs to the Mediator complex. To explore this possibility, we compared the genome occupancy for FLAG-tagged MED25 and ETV4 in the prostate cancer cell line PC3, which overexpresses ETV4. We also tested for relevance of MED25 and ETV4 binding to for gene expression in PC3s. We found a high degree of overlap in the FLAG-MED25 and ETV4 ChIPs datasets consistent with our model, and also identified a subset of target genes co-dependent on Med25 and ETV4.