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Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin [mature pollen]

ABSTRACT: Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin [mature pollen]

PROVIDER: PRJNA573713 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10166243.fastq.gz	Fastqsanger.gz
	SRR10166244.fastq.gz	Fastqsanger.gz
	SRR10166245.fastq.gz	Fastqsanger.gz
	SRR10166246.fastq.gz	Fastqsanger.gz
	SRR10166247.fastq.gz	Fastqsanger.gz

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Similar Datasets

Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin [mature pollen]

Project description:Epigenetic marks are reprogrammed in the gametes to reset genomic potential in the next generation. In most animal species, paternal chromatin is extensively reprogrammed through the global erasure of DNA methylation and the exchange of histones with protamine. Precisely how the paternal epigenome is reprogrammed in flowering plants remains unclear since sperm chromatin is not demethylated and histones are retained. Here, we show that the sperm-specific histone, H3.10, is immune to lysine 27 methylation and that its deposition in sperm contributes to the global and specific resetting of the epigenetic mark H3K27me3 by uncoupling its inheritance during DNA replication. The loss of H3K27me3 facilitates transcription of genes essential for spermatogenesis and pre-configures sperm with a chromatin state that forecasts gene expression in the next generation, revealing a global wave of epigenetic resetting that coordinates with early plant life. Thus, in contrast to the indiscriminate removal of epigenetic marks in animal sperm, plants have evolved a specific mechanism to simultaneously differentiate male gametes and reprogram the paternal epigenome.

2020-03-17 | GSE137887 | GEO

Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin

Project description:Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin

| PRJNA494056 | ENA

Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin

Project description:Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin

| PRJNA494055 | ENA

Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin

Project description:Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin

| PRJNA494054 | ENA

Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin [sperm]

Project description:Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin [sperm]

| PRJNA605233 | ENA

Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin [sperm]

Project description:Epigenetic marks are reprogrammed in the gametes to reset genomic potential in the next generation. In mammals, paternal chromatin is extensively reprogrammed through the global erasure of DNA methylation and the exchange of histones with protamines1,2. Precisely how the paternal epigenome is reprogrammed in flowering plants remains unclear since DNA is not demethylated in sperm and histones are retained3,4. Here, we describe a multi-layered mechanism by which H3K27me3 is globally lost from histone-based sperm chromatin in Arabidopsis. This mechanism involves silencing of H3K27me3 ‘writers’, the activity of H3K27me3 ‘erasers’ and deposition of a sperm-specific histone, H3.105, which we show is immune to lysine 27 methylation. The loss of H3K27me3 facilitates transcription of genes essential for spermatogenesis and pre-configures sperm with a chromatin state that forecasts gene expression in the next generation. Thus, plants have evolved a specific mechanism to simultaneously differentiate male gametes and reprogram the paternal epigenome.

2020-03-17 | GSE144899 | GEO

Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin

2020-03-17 | GSE120668 | GEO

Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin

2020-03-17 | GSE120664 | GEO

Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin [sperm - third party reanalysis]

2020-03-17 | GSE144900 | GEO

Phosphoproteomics profiling of tobacco mature pollen and pollen activated in vitro

Project description:Tobacco mature pollen has extremely desiccated cytoplasm, and is metabolically quiescent. Upon re-hydration it becomes metabolically active and that results in later emergence of rapidly growing pollen tube. These changes in cytoplasm hydration and metabolic activity are accompanied by protein phosphorylation. In this study, we subjected mature pollen, 5-min-activated pollen, and 30-min-activated pollen to TCA/acetone protein extraction, trypsin digestion and phosphopeptide enrichment by titanium dioxide. The enriched fraction was subjected to nLC-MS/MS. We identified 471 phosphopeptides that carried 432 phosphorylation sites, position of which was exactly matched by mass spectrometry. These 471 phosphopeptides were assigned to 301 phosphoproteins, since some proteins carried more phosphorylation sites. Of the 13 functional groups, the majority of proteins were put into these categories: transcription, protein synthesis, protein destination and storage, and signal transduction. Many proteins were of unknown function, reflecting the fact that male gametophyte contains many specific proteins that have not been fully functionally annotated. The quantitative data highlighted the dynamics of protein phosphorylation during pollen activation; the identified phosphopeptides were divided into seven groups based on the regulatory trends. The major group comprised mature pollen-specific phosphopeptides that were dephosphorylated during pollen activation. Several phosphopeptides representing the same phosphoprotein had different regulation, which pinpointed the complexity of protein phosphorylation and its clear functional context. Collectively, we showed the first phosphoproteomics data on activated pollen where the position of phosphorylation sites was clearly demonstrated and regulatory kinetics was resolved.

2016-01-27 | PXD003042 | Pride

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