Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A

ABSTRACT: GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A

PROVIDER: PRJNA574941 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10202600.fastq.gz	Fastqsanger.gz
	SRR10202601.fastq.gz	Fastqsanger.gz
	SRR10202602.fastq.gz	Fastqsanger.gz
	SRR10202597.fastq.gz	Fastqsanger.gz
	SRR10202598.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A

Project description:Excessive expression of subunit 1 of GIRK1 in ER+ breast tumors is associated with reduced survival times, and increased lymph node metastasis in patients. To investigate possible tumor-initiating properties, benign MCF10A and malign MCF7 mammary epithelial cells overexpressing GIRK1 were engineered, neoplasia associated vital parameters and resting potentials were measured and compared to controls. Presence of GIRK1 resulted in resting potentials negative to controls. Upon GIRK1 overexpression, several cellular pathways were sizably regulated towards pro-tumorigenic action as revealed by comparison of transcriptomes of MCF10AGIRK1 with control (MCF10AeGFP). According to transcriptome analysis, cellular migration was promoted while wound healing and extracellular matrix interactions were impaired. Vital parameters in MCF7 cells were affected akin the benign MCF10A lines, although to a lesser extent. Thus, GIRK1 overexpression will aid in classification of ER+ breast tumors and bears the potential to open a new window for the therapy of this detrimental disease.

2019-10-01 | GSE138155 | GEO

GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A.

Project description:Excessive expression of subunit 1 of GIRK1 in ER+ breast tumors is associated with reduced survival times and increased lymph node metastasis in patients. To investigate possible tumor-initiating properties, benign MCF10A and malign MCF7 mammary epithelial cells were engineered to overexpress GIRK1 neoplasia associated vital parameters and resting potentials were measured and compared to controls. The presence of GIRK1 resulted in resting potentials negative to the controls. Upon GIRK1 overexpression, several cellular pathways were regulated towards pro-tumorigenic action as revealed by comparison of transcriptomes of MCF10AGIRK1 with the control (MCF10AeGFP). According to transcriptome analysis, cellular migration was promoted while wound healing and extracellular matrix interactions were impaired. Vital parameters in MCF7 cells were affected akin the benign MCF10A lines, but to a lesser extent. Thus, GIRK1 regulated cellular pathways in mammary epithelial cells are likely to contribute to the development and progression of breast cancer.

| S-EPMC6917815 | biostudies-literature

5AZA-induced Gene Expression in Human Breast Cancer Cell Lines and Benign Primary Mammary Epithelial Cell Cultures

Project description:The goal of this experiment was to identify genes that were expressed at higher levels in benign human mammary epithelial cells than in breast cancer cell lines and that were induced by 5AZA treatment in breast cancer cell lines. Six breast cancer cell lines were selected for demethylation studies based on known tumor suppressor gene expression regulation by promoter region hypermethylation: HCC1569 (CCND2), HCC1954 (SCGB3A1, APC, RASSF1A), MCF-7 (RAR-beta2), MDA-MB-231 (ESR1), UACC3199 (BRCA1), and BT-549 (hypermethylator phenotype). Other than MCF10A we specifically avoided immortalized benign human mammary epithelial cell lines for this experiment as these cells frequently show tumor suppressor gene methylation (e.g. p16) and gene expression profiles that are intermediate between normal breast epithelial cells and breast cancer. Instead, we opted to test six first-passage benign human mammary epithelial cell cultures (HME) generated in serum-free media from small fragments of normal breast tissue obtained from young women undergoing fibroadenoma excision. The 5AZA dose (0.5 microM) was selected based on evaluation of growth curves and induction of BNC1, SERPINB, and TKTL1 gene expression measured by RT-PCR in benign and malignant cells. The breast cancer cell lines, HME cultures, and MC10A cells were treated with 0.5 microM 5AZA (Sigma-Aldrich, St. Louis, MO) in DMSO or DMSO alone for six days after which the cells were harvested, and RNA prepared using the Illumina TotalPrep kit (AMIL1791, Life Technologies, Grand Island, NY). Whole genome expression was assessed using the Illumina HumanWG-6-v3 chip Gene expression was evaluated in 6 breast cancer cell lines, 6 primary breast epithelial cell cultures, and MCF10A cells after 6 days in DMSO or DMSO plus 0.5 microM 5AZA.

2012-10-19 | E-GEOD-41692 | biostudies-arrayexpress

5AZA-induced Gene Expression in Human Breast Cancer Cell Lines and Benign Primary Mammary Epithelial Cell Cultures

2012-10-19 | GSE41692 | GEO

BRCA1 mutation influences progesterone response in human benign mammary organoids.

Project description:BRCA1 mutation influences progesterone response in human benign mammary organoids.

| PRJNA544257 | ENA

BRCA1 mutation influences progesterone response in human benign mammary organoids.

Project description:Mammary organoids treated with a 28 day menstrual cycle profile of estradiol and progesterone with or without TPA: Comparison of normal versus BRCA1 mut cells. Purpose: The goals of this study are to determine 1) the effects of a 28 day menstrual cycle profile of estradiol and progesterone, 2) the effects of TPA, and 3) the responses in normal versus BRCA1 carriers, in benign breast organoids using RNA-seq

2019-12-10 | GSE131640 | GEO

Gene expression profiles induced by overexpression of PDEF in MCF10A mammary epithelial cell line

Project description:Recent studies suggest that PDEF is required for secretory cell differentiation in several epithelial tissues. To investigate PDEF in the mammary gland, we examined the effect of this transcription factor on gene expression using microarray based profiling of MCF-10A cells. These cells are non-transformed mammary epithelial cells that express protein and gene expression programs of basal epithelial cells and undetectable levels of endogenous PDEF. Bioinformatics analysis of the genes induced or repressed by PDEF overexpression in MCF10A cells revealed a striking effect on expression of luminal and myoepithelial cell markers.

2014-03-29 | GSE40986 | GEO

RNAseq transcriptomic data of the human mammary cell line MCF10A expressing a shRNA against MME (CD10) or a control shRNA

Project description:The immortalized but not transformed human mammary epithelial stem-like cell line MCF10A was stably transfected with a lentiviral vector expressin a shRNA targeting the MME transcript, coding for the CD10 membrane endopeptidase. This study aims to identify genes regulated by the CD10 modulation.

2023-09-01 | GSE186735 | GEO

MUC1 triggers lineage plasticity of Her2 positive mammary tumor

Project description:MUC1 triggers lineage plasticity of Her2 positive mammary tumor

| PRJNA814266 | ENA

Differential microRNA expression in adherent versus suspended MCF10A mammary epithelial cells

Project description:Differential microRNA expression in adherent versus suspended MCF10A mammary epithelial cells

| PRJNA1025018 | ENA