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ABSTRACT: Bacterial community of Yerba Mate plants after inoculation with Kosakonia radicincitans YD4

PROVIDER: PRJNA575649 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10224437_1.fastq.gz	Fastqsanger.gz
	SRR10224437_2.fastq.gz	Fastqsanger.gz
	SRR10224438_1.fastq.gz	Fastqsanger.gz
	SRR10224438_2.fastq.gz	Fastqsanger.gz
	SRR10224439_1.fastq.gz	Fastqsanger.gz

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Project description:Community structure and diversity of the root-associated microbiome of Ilex paraguariensis St. Hil (Yerba Mate) after bio-inoculation with Kosakonia radicincitans YD4

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Modulation of the endophytic strain Kosakonia radicincitans UYSO10 proteome by sugarcane roots exudate

Project description:The objective of this work was to expand the knowledge about the possible mechanisms involved in the early stages of the interaction between the diazotrophic endophytic strain Kosakonia radicincitans UYSO10 and sugarcane plants. For that, a proteomic approach was conducted in the strain UYSO10 exposed or not to sugarcane exudates. Results showed that in the presence of root exudates the UYSO10 strain senses the environment and adapts its proteome to transport and metabolize different nutrients, and to interact with the host plant. These results deepen the knowledge of the potential mechanisms involved in the early stage of plant-bacteria endophyte interaction.

2024-12-03 | PXD050409 | Pride

Gene expression data from yerba mate treated and non-treated cultured PBMCs activated with phytohemagglutinin

Project description:Yerba mate (YM) has been shown to have anti-inflammatory properties in several studies. However, this effect has been found mainly in obesity-related in inflammation. The aim of this work was to study the effect of YM in cultured peripheral blood mononuclear cells to see whether it has anti-inflammatory properties. We stimulated peripheral blood mononuclear cells in vitro with phitohemaglutinin in the presence of yerba mate and determined their activation measuring the the expression of CD25 by flow cytometry. We observed that YM treatment produced a dose-dependent reduction in PBMC activation (CD25 positive cells) when they were stimulated with PHA. This effect was also observed in T cells (CD3 positive) subpopulation. Microarray analysis revealed the differential expression of 128 genes in YM-treated cells. According to a protein-protein interaction database, these genes were highly connected and they are involved in inflammatory response. In summary, it was demonstrated that YM produces a reduction in the amount of activated cells under the stimulation of PHA. Therefore, it might be used in diseases with an inflammatory component. PBMC samples from a healthy individual were cultured and stimulated with phytohemagglutinin. Gene expression of yerba mate treated and non-treated cells was analyzed in duplicates, having in total four microarrays.

2016-08-15 | E-GEOD-84407 | biostudies-arrayexpress

Kosakonia radicincitans CC111

Project description:Kosakonia radicincitans CC111 genome sequencing

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Kosakonia radicincitans strain:GXGL-4A

Project description:Kosakonia radicincitans complete genome sequencing

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Gene expression data from yerba mate treated and non-treated cultured PBMCs activated with phytohemagglutinin

2016-08-15 | GSE84407 | GEO

Computational Ranking of Yerba Mate Small Molecules Based on Their Predicted Contribution to Antibacterial Activity against Methicillin-Resistant Staphylococcus aureus

Project description:The aqueous extract of yerba mate, a South American tea beverage made from Ilex paraguariensis leaves, has demonstrated bactericidal and inhibitory activity against bacterial pathogens, including methicillin-resistant Staphylococcus aureus (MRSA). The gas chromatography-mass spectrometry (GC-MS) analysis of two unique fractions of yerba mate aqueous extract revealed 8 identifiable small molecules in those fractions with antimicrobial activity. For a more comprehensive analysis, a data analysis pipeline was assembled to prioritize compounds for antimicrobial testing against both MRSA and methicillin-sensitive S. aureus using forty-two unique fractions of the tea extract that were generated in duplicate, assayed for activity, and analyzed with GC-MS. As validation of our automated analysis, we checked our predicted active compounds for activity in literature references and with used authentic standards to test for antimicrobial activity. 3,4-dihydroxybenzaldehyde showed the most antibacterial activity against MRSA at low concentrations in our bioassays. In addition, quinic acid and quercetin were identified using random forests analysis and 5-hydroxy pipecolic acid was identified using linear discriminant analysis. We additionally also generated a ranked list of unidentified compounds that may contribute to the antimicrobial activity of yerba mate against MRSA. Here we utilized GC-MS data to implement an automated analysis that resulted in a ranked list of compounds that likely contribute to the antimicrobial activity of aqueous yerba mate extract against MRSA.

2015-03-19 | MTBLS170 | MetaboLights

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Project description:Kosakonia radicincitans strain:DSM 107547 Genome sequencing

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Project description:Kosakonia radicincitans strain:dy20221129 Genome sequencing and assembly

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