Project description:Pseudomonas syringae pv. actinidiae (Psa) is responsible for causing kiwifruit canker disease, which severely impacts on the global kiwifruit industry, leading to substantial economic losses. Cooler environmental temperatures (below 20℃) are known to favor the outbreak of this bacterial disease in the field. However, the specific mechanisms that enable Psa to adapt and thrive in such conditions have remained elusive. In this study, we discovered that the gene RS16350, encoding a heat shock protein, positively regulates pathogenicity in kiwifruit leaves at low temperature (16℃), but not at room temperature (28℃). Delving into the mechanics, we found that RS16350 directly interacts with HrpL, an RpoN-dependent RNA polymerase sigma factor that serves as a master regulator of the type III secretion system (T3SS) in Psa. This RS16350-HrpL interaction enhances HrpL’s binding affinity to hrp-box, which in turn elevates the expression of downstream T3SS genes, thereby boosting virulence. Consequently, we have designated RS16350 as TspR (temperature sensitive and pathogenic regulator). Our findings shed light on how a kiwi pathogen enhances its virulence in response to low temperatures, paving the way for the development of innovative strategies to combat kiwifruit canker disease.
Project description:Thermal condition is an important envrionment factor to produce healthy pig. A mineral heat ware, RAVI is a regenerative far-infrared heating system. The purpose of this sutyd was to evaluate immunostimulating effects in pigs when RAVI was applied to maintain thermal condition compared with gasoline heater.
2019-05-29 | GSE131821 | GEO
Project description:MRSA outbreak in a tertiary neonatal intensive care unit in Iceland
Project description:Human A549 lung epithelial cells were exposed directly at the air-liquid interphase towards combustion aerosols of wood burning. The goal was to compare the responses towards different wood and burning conditions. Beech log woods were burnt in a modern masonry heater, soft wood pellets were burnt in a pellet boiler.