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Transcriptomic changes upon overexpression of S. cerevisiae Ppz1 protein phosphatase

ABSTRACT: Transcriptomic changes upon overexpression of S. cerevisiae Ppz1 protein phosphatase

PROVIDER: PRJNA577044 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10260800.fastq.gz	Fastqsanger.gz
	SRR10260801.fastq.gz	Fastqsanger.gz
	SRR10260784.fastq.gz	Fastqsanger.gz
	SRR10260785.fastq.gz	Fastqsanger.gz
	SRR10260786.fastq.gz	Fastqsanger.gz

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Transcriptomic changes upon overexpression of S. cerevisiae Ppz1 protein phosphatase

Project description:Overexpression of the protein phosphatase Ppz1 is toxic for yeast cells as it blocks cell cycle progression from G1 to S phase. To identify relevant changes caused by Ppz1 overexpression we have constructed a strain (ZCZ01) in wich the PPZ1 promoter has been replaced by the GAL promoter. Cell grown on raffinose were shifted to galactose and samples were taken after 30, 120 and 240 min and changes in mRNA levels analyzed by RNA-seq.

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