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RNA-Seq to evaluate detaining intron splicing upon O-GlcNAc perturbation

ABSTRACT: RNA-Seq to evaluate detaining intron splicing upon O-GlcNAc perturbation

PROVIDER: PRJNA577128 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10268100_1.fastq.gz	Fastqsanger.gz
	SRR10268100_2.fastq.gz	Fastqsanger.gz
	SRR10268101_1.fastq.gz	Fastqsanger.gz
	SRR10268101_2.fastq.gz	Fastqsanger.gz
	SRR10268102_1.fastq.gz	Fastqsanger.gz

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RNA-Seq to evaluate detaining intron splicing upon O-GlcNAc perturbation

Project description:We report the effect of splicing upon O-GlcNAc perturbation with OGT inhibitor or OGA inhibitor. We found that splicing is acutely affected through our phosphoproteomics data when cells are treated with OGT inhibitor. By obtaining the various splicing events that are affected by OGT or OGA inhibition, we found that O-GlcNAc is a major regulator of detained introns splicing. At early time point, we observed highly specific splicing of OGT/OGA detained introns to buffer O-GlcNAc changes. At longer time point, ~80% of the total detained introns are affected by O-GlcNAc perturbation, while the rest of canonical splicing events are only minimally affected (~ 5%).

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SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing [CRISPR]

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