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Host metabolic reprogramming during immune activation promotes intracellular bacterial survival (2DG)

ABSTRACT: Host metabolic reprogramming during immune activation promotes intracellular bacterial survival (2DG)

PROVIDER: PRJNA578745 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10320575_1.fastq.gz	Fastqsanger.gz
	SRR10320575_2.fastq.gz	Fastqsanger.gz
	SRR10320576_1.fastq.gz	Fastqsanger.gz
	SRR10320576_2.fastq.gz	Fastqsanger.gz
	SRR10320577_1.fastq.gz	Fastqsanger.gz

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Host metabolic reprogramming during immune activation promotes intracellular bacterial survival (2DG)

Project description:Intracellular pathogens, such as Salmonella enterica serovar Typhimurium (S.Tm), are able to sense and respond to a changing host cell environment. Macrophages exposed to microbial products undergo metabolic changes that are increasingly understood to drive a productive inflammatory response. However, the role of macrophage metabolic reprogramming in bacterial adaptation to the intracellular environment has not been explored. Here we show that changes in host metabolic state serve as a signal detected byS.Tm. Using metabolic profiling and dual RNA-seq, we show that succinate accumulates in infected macrophages and is sensed by intracellular S.Tm to promote induction of virulence genes. Succinate uptake by the bacterium drives induction of pmrAB-dependent genes and SPI-2 virulence-associated regulon. S.Tm lacking the DcuB transporter for succinate uptake display impaired intracellular survival. Our work demonstrates that accumulation of metabolic intermediates, necessary for macrophage activation, promote intracellular survival of pathogens, opening a new realm of metabolic host-pathogen crosstalk.

2022-10-19 | GSE139206 | GEO

Host metabolic reprogramming during immune activation promotes intracellular bacterial survival (host and bacteria)

2022-10-19 | GSE139205 | GEO

Host metabolic reprogramming during immune activation promotes intracellular bacterial survival (glycerol or succinate)

2022-10-19 | GSE139207 | GEO

Host metabolic reprogramming during immune activation promotes intracellular bacterial survival (host and bacteria)

Project description:Host metabolic reprogramming during immune activation promotes intracellular bacterial survival (host and bacteria)

| PRJNA578744 | ENA

Host metabolic reprogramming during immune activation promotes intracellular bacterial survival (glycerol or succinate)

Project description:Host metabolic reprogramming during immune activation promotes intracellular bacterial survival (glycerol or succinate)

| PRJNA578747 | ENA

Transcriptional Profiling of high pigment-2dg Tomato Mutant Fruit

Project description:Phenotypes of the tomato (Solanum lycopersicum L.) high pigment-2dg (hp-2dg) mutant are caused by a mutation in the gene encoding DEETIOLATED1, a negative regulator of light signaling. Homozygous hp-2dg plants display a plethora of distinctive developmental and metabolic phenotypes in comparison to their normal isogenic counterparts. This mutant is however best known for the increased levels of lycopene and other plastid-accumulating functional metabolites. In this study we analyzed the transcriptional alterations in mature-green, breaker and early-red fruits of hp-2dg/hp-2dg plants in relation to their normal counterparts using microarray technology. Results show that a large portion of the genes that are affected by hp-2dg mutation, display a tendency for up- rather than down-regulation. Ontology assignment of these differentially regulated transcripts, revealed a consistent upregulation of those related to chloroplast biogenesis and photosynthesis in hp-2dg mutants throughout fruit ripening. A tendency of upregulation was also observed in structural genes involved in phytonutrient biosynthesis. However, this upregulation was not as consistent, positioning plastid biogenesis as an important determinant of phytonutrient overproduction in hp-2dg mutant fruits. Microscopic observations revealed a highly significant increase in chloroplasts size and number in pericarp cells of mature-green hp-2dg/hp-2dg fruits in comparison to their normal counterparts. This increase could be observed from early stages of fruit development. Therefore, the molecular trigger that drives phytonutrient overproduction in hp-2dg mutant fruits should be initially traced at early stages of fruit development. Keywords: Comparative Transcriptional Profiling of tomato fruit pericarp tissue

2007-10-08 | GSE6041 | GEO

A host-directed adjuvant sensitizes intracellular bacterial persisters to antibiotics

Project description:Intracellular bacterial reservoirs contribute to antibiotic treatment failure by fostering metabolically dormant persister cells that are highly tolerant to killing. However, strategies to effectively target intracellular persister cells remain limited. Here, we developed a high-throughput screen to identify compounds that modulate the metabolic activity of intracellular Staphylococcus aureus. The identified compound, KL1, increases intracellular bacterial metabolic activity and sensitizes persister populations of S. aureus to antibiotics, without causing cytotoxicity or bacterial outgrowth. KL1 also exhibits adjuvant activity against intramacrophage Salmonella enterica Typhimurium and Mycobacterium tuberculosis, as well as in murine infection models of S. aureus and S. Typhimurium infection. Transcriptomic analysis and further mechanistic studies reveal that KL1 modulates host immune response genes and suppresses the production of reactive species in host macrophages, alleviating a key inducer of antibiotic tolerance. Our findings highlight the potential to target intracellular persisters by stimulating their metabolism.

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Differentially expressed genes in iKRAS cell treated with Trametinib, 2DG and Combo

Project description:To identify the differentially expressed gene in ikras cells treated with Trametinib, 2DG and Trametinib/2DG combination. Two ikras cells were used for transcriptomics analysis by RNAseq.

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Nutritional immunity promotes intracellular survival of nontypeable Haemophilus influenzae

Project description:Nutritional immunity promotes intracellular survival of nontypeable Haemophilus influenzae via altered intracellular trafficking

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Project description:Akkermansia muciniphila-induced trained immune phenotype increases bacterial intracellular survival and attenuates inflammation

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