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MRNA analysis in depolarized neuroblastoma cells

ABSTRACT: mRNA analysis in depolarized neuroblastoma cells

PROVIDER: PRJNA579890 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10353652.fastq.gz	Fastqsanger.gz
	SRR10353653.fastq.gz	Fastqsanger.gz
	SRR10353654.fastq.gz	Fastqsanger.gz
	SRR10353655.fastq.gz	Fastqsanger.gz
	SRR10353656.fastq.gz	Fastqsanger.gz

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mRNA analysis in depolarized neuroblastoma cells

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CircRNA analysis in depolarized neuroblastoma cells

Project description:We profiled circRNA using RNA-seq in the depolarized neuroblastoma cells to detect depolarization associated circRNAs

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High-throughput sequencing Analysis of Transcriptomes of depolarized mouse BMDM cell

Project description:Purpose:Mouse BMDM is the universal cell type of studying innate immunity.This study was to analyze LPS induced innate immune respone and the relationship between the membrane potential and LPS induced innate immune respone Methods: WT BMDM cells were treated with or without LPS for 6 hours in the pressence of membranepotential depolarization stimuli. Then mRNA profiles of these samles were generated by High-throughput sequencing analysis, in triplicate, using Illumina HiSeq /Novaseq or MGI2000. And the differencial mRNA profiles were analyzed. Results: mRNA profiles of more than 20000 genes were analyzed, and differencial expression profiles were compared . Conclusions: Our study represents the first detailed analysis of transcriptomes of WT and membrane potential depolarized macrophages, with biologic replicates, generated by RNA-seq technology. This data was useful to analyze the relationship between membrane potential and LPS induced innate immune respone

2022-04-05 | GSE183067 | GEO

Analysis of circRNA,mRNA and miRNA in depolarized neuroblastoma cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-03-06 | GSE139443 | GEO

Polysome profiling of mRNA translation in neuroblastoma cells in response to asparagine/alanine supplement

Project description:Neuroblastoma, the most common extracranial solid malignancy in infants and young children, originates from aberrant neural crest cells and accounts for 10-15% of childhood cancer deaths. However, the underlying mechanisms regulating neuroblastoma progression remain to be determined. To investigate the mechanisms regulating tumorigenesis and aggressiveness, we employed the Illumina HiSeq X Ten as a discovery platform to analyze the polysome profiling of mRNA translation in neuroblastoma cells in response to asparagine/alanine (Asn/Ala) supplement. The results showed that 618 and 566 translating mRNAs were respectively elevated or reduced in SK-N-BE(2) after Asn/Ala treatment. Furthermore, we validated the polysome profiling results by western blot with high identity. Overall, our results provided fundamental information about the mRNA translation changes in response to Asn/Ala treatment in human neuroblastoma cells, and these findings will help us understand the pathogenesis of neuroblastoma progression.

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Analysis of Nucleocytoplasmic Protein Separation in DPST-Crosslinked Human Neuroblastoma SY5Y Cells

Project description:Following DPST crosslinking of human neuroblastoma cells, nuclear-cytoplasmic fractionation was performed using hypotonic lysis to obtain cytoplasmic and nuclear protein compartments. Through systematic analysis of crosslinking mass spectrometry data, we elucidated how subcellular compartmentalized microenvironments regulate spatial distribution of protein conformations and topological architecture of interaction networks. This study reveals the heterogeneous principles governing dynamic protein assembly between nuclear and cytoplasmic compartments in neuroblastoma cells.

2025-04-14 | PXD062913 |

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