Project description:To determine whether and how warming affects the functional capacities of the active microbial communities, GeoChip 5.0 microarray was used. Briefly, four fractions of each 13C-straw sample were selected and regarded as representative for the active bacterial community if 16S rRNA genes of the corresponding 12C-straw samples at the same density fraction were close to zero.
Project description:Shallow-water ascidians are prevalent invasive and fouling organisms within marine ecosystems. Their larvae undergo rapid metamorphosis from planktonic to sessile stage, facilitating colonization and population expansion. It is crucial to explore the molecular and cellular orchestration of larval metamorphosis in order to gain a deep understanding of ascidian environmental adaptability, as well as to develop effective methods for bioinvasion and biofouling control. Here, we conducted 4D-label free quantitative proteomics combined with transcriptomics to monitor protein and gene expression across five distinct developmental stages from swimming larva to metamorphic juvenile of ascidian Ciona. We identified a total of 6,043 proteins, approximately 83.6% of which were quantified. We delineated the temporal dynamics of protein expression during ascidian metamorphosis and identified differentially expressed proteins between stages.