Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chromatin-associated RNA of HSV-1 infected primary human fibroblasts including infection with vhs mutants

ABSTRACT: Chromatin-associated RNA of HSV-1 infected primary human fibroblasts including infection with vhs mutants

PROVIDER: PRJNA588169 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10416485.fastq.gz	Fastqsanger.gz
	SRR10416486.fastq.gz	Fastqsanger.gz
	SRR10416487.fastq.gz	Fastqsanger.gz
	SRR10416488.fastq.gz	Fastqsanger.gz
	SRR10416489.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

Chromatin-associated RNA of HSV-1 infected primary human fibroblasts including infection with vhs mutants

Project description:Primary human foreskin fibroblasts (HFF) were infected with wild-type herpes simplex virus 1 (HSV-1) strain 17 or its vhs null mutant, or with BAC-derived viruses expressing either a wild-type or catalytically inactive vhs with the D195N mutation at a multiplicity of infection (MOI) of 10. Chromatin-associated RNA was prepared and subjected to RNA-seq.

2020-10-26 | GSE140068 | GEO

Subcellular RNA fractions of HSV-1 infected primary human fibroblasts including infection with delta-Vhs mutant

Project description:Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 or its vhs null mutant at a multiplicity of infection (MOI) of 10. Subcellular RNA fractions including total RNA, cytoplasmic RNA, nucleoplasmic RNA and chromatin-associated RNA were prepared and subjected to RNA-seq.

2020-10-26 | GSE129582 | GEO

Subcellular RNA fractions of HSV-1 infected primary human fibroblasts including infection with delta-Vhs mutant

Project description:Subcellular RNA fractions of HSV-1 infected primary human fibroblasts including infection with delta-Vhs mutant

| PRJNA531885 | ENA

Total and newly transcribed (4sU-RNA) RNA of primary human fibroblasts infected with the vhs-null mutant of HSV-1

Project description:Primary human foreskin fibroblasts (HFF) were infected with vhs-null mutant of HSV-1 strain 17 at a multiplicity of infection (MOI) of 10. Newly transcribed RNA was labelled by adding 500µM 4-thiouridine (4sU) to the cell culture media for 1h. Total cellular RNA was isolated using Trizol. Newly transcribed RNA was purified following the protocol described in Raedle et al. JoVE 2013.

2020-10-26 | GSE129715 | GEO

Subcellular RNA fractions of HSV-1 infected primary human fibroblasts

Project description:Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. Subcellular RNA fractions including total RNA, cytoplasmic RNA, nucleoplasmic RNA and chromatin-associated RNA were prepared and subjected to RNA-seq

2018-03-08 | GSE100576 | GEO

HSV-1 interferes with STAT3 expression in infected mature dendritic cells

Project description:Herpes simplex virus type 1 (HSV-1) is the prototype of the α-herpesvirus family. To propagate within the host organism, HSV-1 has evolved several strategies to subvert the host immune response. Due to the pivotal role of dendritic cells (DCs), bridging innate and adaptive immunity and activating naïve T cells, they represent an attractive target for HSV-1-triggered immune regulation. Here, we report a novel HSV-1-mediated mechanism of signal transducer and activator of transcription 3 (STAT3) dysregulation in human monocyte-derived mature DCs (mDCs). Due to STAT3’s antiviral activity, STAT3 was shown to be modulated by viruses to efficiently replicate in several different cell types, however, not in DCs. We show that HSV-1 infection of mDCs leads to diminished total STAT3 expression and STAT3 phosphorylation (pSTAT3), as well as downregulation of STAT3 mRNA very early upon infection. Protein downregulation of STAT3 could be verified by label-free mass spectrometric analysis of HSV-1- and HSV-2-infected mDCs. We found that two viral proteins, the tegument protein virion host shutoff (vhs) and the immediate early (IE) protein ICP27, contribute to STAT3 protein downregulation upon HSV-1 infection. However, HSV-1 Δvhs and ΔICP27 deletion strains still inhibited pSTAT3 in infected mDCs, suggesting that different viral proteins impair STAT3 phosphorylation and total STAT3. Moreover, STAT3 protein levels were degraded in a proteasome-dependent, but apoptosis-independent manner. Taken together, we demonstrate that HSV-1 downregulates STAT3 in HSV-1-infected human mDCs at the transcript, protein and phosphorylation levels. Downregulation of STAT3 protein depended on vhs and the proteasome, very likely to shape DC function for immune evasion and modulation.

2026-07-23 | PXD081503 | Pride

Total and newly transcribed (4sU-RNA) RNA of primary human fibroblasts infected with the vhs-null mutant of HSV-1

Project description:Total and newly transcribed (4sU-RNA) RNA of primary human fibroblasts infected with the vhs-null mutant of HSV-1

| PRJNA532584 | ENA

HSV-1 interferes with STAT3 expression in infected mature dendritic cells. Part 1

2026-07-21 | PXD081194 | Pride

HSV-1 interferes with STAT3 expression in infected mature dendritic cells. Part 3

2026-07-22 | PXD081438 | Pride

H3K4me3 ChIPmentation on HSV-1 infected primary human fibroblasts

Project description:Primary human fetal foreskin fibroblasts (HFFFs) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. ChIPmentation libraries were prepared starting with 500,000 cells per condition following the protocol described by Christian Schmidl et al, Nature Methods 2015

2020-10-26 | GSE132920 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data