Project description:This study generated 10x Genomics Visium spatial transcriptomic profiles from mouse liver sections to investigate the effects of high-fat diet and kimchi supplementation on hepatic gene expression organization. Samples were obtained from male C57BL/6J mice assigned to normal diet (ND), high-fat diet (HFD), high-fat diet supplemented with spontaneously fermented kimchi (S-K), and high-fat diet supplemented with starter kimchi fermented with Leuconostoc mesenteroides KCKM0828 (LMS-K). The dataset was designed to assess diet-associated transcriptional changes across the liver lobule and to evaluate how kimchi intervention modulates zonation-related hepatic programs along the portal-to-central axis. Raw FASTQ files were prepared for SRA submission through GEO, and processed outputs include filtered gene-barcode matrices, HDF5 matrices, spatial coordinate files, scale factor files, tissue images, and summary reports.
Project description:We report here NGS RNA-seqencing datasets with wild-type (Chiifu) and kimchi cabbage with Arabidopsis thaliana Phytochrome B (named AtPHYB) grown under optimal temperature (22℃) condition.
Project description:This study examines the role of early exposure to gut microbes and poor diet on microglial function in mice. Groups = control (CON), malnourished (MAL), and malnourished + microbial exposure (E/MALBG). CD11b+ cells (microglial enrichment) were isolated from whole mouse brains (Adult Brain Disruption Kit, Miltenyi Biotec). After sample quality control (Agilent 2100 Bioanalyzer), qualifying samples were sent for RNA-Seq (Illumina NextSeq 500 with Paired End 42bp × 42bp reads; demultiplexed: Illumina's bcl2fastq2). Following alignment against mouse reference genes (STAR aligner), DEG analyses was conducted using the DESeq2 pipeline.