Project description:17β-estradiol (E2) pollution has attracted much attention, and the existence of E2 poses certain risks to the environment and human health. However, the mechanism of microbial degradation of E2 remains unclear. In this study, the location of E2-degrading enzymes was investigated, and transcriptome analysis of Microbacterium resistens MZT7 (M. resistens MZT7) exposed to E2. The degradation of E2 by M. resistens MZT7 was via the biological action of E2-induced intracellular enzymes. With the RNA sequencing, we found 1,109 differentially expressed genes (DEGs). Among, 773 genes were up-regulated and 336 genes were down-regulated. The results of the RNA sequencing indicated the DEGs were related to transport, metabolism, and stress response. Genes for transport, transmembrane transport, oxidoreductase activity, ATPase activity, transporter activity and quorum sensing were up-regulated. Genes for tricarboxylic acid cycle, ribosome, oxidative phosphorylation and carbon metabolism were down-regulated. These findings provide some new insights into the molecular mechanism of biotransformation of E2 by M. resistens MZT7.
Project description:Drought is one of the most detrimental environmental factors that adversely affect crop production, thus jeopardizing food supplies for a growing world population. Over the past years, it has become evident that microorganisms associated with plants can enhance drought tolerance. However, the specific genetic and molecular mechanisms underpinning bacterial induction of drought tolerance in plants are still largely unknown. In our work, we have shown that a root endophytic Flavobacterium sp. 98 confers significant drought tolerance to Arabidopsis thaliana without compromising growth and yield. Here, we compared the transcriptome of Arabidopsis seedlings inoculated with Flavobacterium or mock (time series) to identify transcription reprograming induced by Flavo in plants.
