Project description:High-throughput RNA sequencing were performed to identify a large number of mRNAs in developmental herbaceous peony seeds at different periods to provide useful information for elucidating the molecular mechanism of the lipid biosynthesis and fatty acid accumulation for herbaceous peony 'Hangshao'.
Project description:Tree peony (Paeonia ostii section Moutan DC.) is known for its excellent ornamental and medicinal values. In 2011, seeds from P. ostii have been identified as novel resource of alpha-linolenic acid (ALA) for seed oil production and development in China. However, the molecular mechanism on biosynthesis of unsaturated fatty acids in tree peony seeds remains unknown. Therefore, transcriptome data is needed to better understand the underlying mechanisms. In this study, lipids accumulation contents were measured using GC-MS methods across developing tree peony seeds, which exhibited an extraordinary ALA content (49.3%) in P. ostii mature seeds. Transcriptome analysis was performed using Illumina sequencing platform. A total of 144 million 100-bp paired-end reads were generated from six libraries, which identified 175,874 contigs. In the KEGG Orthology enrichment of differentially expressed genes, lipid metabolism pathways were highly represented categories. Using this data we identified 388 unigenes that may be involved in de novo fatty acid and triacylglycerol biosynthesis. In particular, three unigenes (SAD, FAD2 and FAD8) encoding fatty acid desaturase with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified.
Project description:This study intends to improve the induction rate of peony seedlings by using different anti-browning agents and different combinations, and clarify the relationship between anti-browning agents and seedling rooting through transcriptome sequencing methods.
2021-12-24 | GSE192345 | GEO
Project description:Identification and functional verification of PlFT gene associated with flowering in herbaceous peony based on transcriptome analysis
Project description:Analyze the different proteins in the pistil after the compatible and incompatible pollination of peony by iTRAQ. The number of identified proteins was 2900, 383 proteins of which were up-regulated expression, and 302 proteins were down-regulated. Those proteins mainly attended the metabolic pathways, biosynthesis of secondary metabolites, ribosome, protein processing in endoplasmic reticulum, spliceosome, RNA transport, glycolysis/gluconeogenesis etc., showed that the proteins and energy metabolism were active. Among them, the RNA degradation, calcium signaling pathway, MAPK signaling pathway, and phosphatidylinositol signaling system were involved in the cross-incompatibility between herbaceous peony and tree peony. Those showed that the energy synthesis of the growing pollen tubes and the polaized growth of pollen tubes were inhibited. Among Enolase, DnaK(HSP70), CALM were down regulating expression, and ANT was up regulating expression.
