Mango leaf compost metagenome
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA589949 | ENA |
REPOSITORIES: ENA
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Project description:Purpose: this study provided a comprehensive sequence for a systemic view of the transcriptome between mango leaf and fruit, as well as fruit allergens, which will be useful for further genomic research studies and breeding of lower allergenic mango cultivars. Methods:Some allergens have previously been identified in mango (Mangifera indica Linn), including profilins, Bet v 1-like proteins and chitinase. In this paper, 66 potential allergen genes were identified and their relative expressions evaluated in mango fruit and leaf using Illumina RNA-Seq technology. Results:A total of 17.63Gb Clean Data was obtained.The number of %≥Q30 was above 94.58%.RNA-Seq generated 11,751,123 contigs that were assembled into 99,328 unigenes with 16,848 unigenes of >1000 bp. A total of 230,242 unigenes were annotated using public protein databases, with a cut-off E-value above 10−5, of which 27,295, 46,030, 24,227 and 14,023 unigenes were assigned to gene ontology terms, Nr, Swiss-Prot and clusters of orthologous groups, respectively. Allergens mainly belonged to pollen allergen, pathogenesis-related protein Bet v I family and NADPH-dependent FMN reductase.
2020-12-01 | GSE142427 | GEO
Project description:The apple mango (Mangifera indica L.) is primarily consumed for its fruit, and the other components of the plant, including the leaves, are often discarded as agricultural by-products. This study investigated the immunomodulatory properties of apple mango leaf extract (AMLE) using transcriptomic and chromatin accessibility analyses, focusing on gene regulation and signaling pathways in bone marrow-derived dendritic cells (BMDCs). Weighted gene co-expression network analysis (WGCNA) revealed two inversely regulated gene network modules. The upregulated module following AMLE treatment included pathways essential for dendritic cell (DC) activation, while the downregulated module was enriched in cell cycle and metabolic pathways. Assay for Transposase Accessible Chromatin with Sequencing (ATAC-seq) analysis revealed increased promoter accessibility in AMLE-treated BMDCs, suggesting increased transcriptional activity. By integrating RNA-seq and ATAC-seq data, we identified 33 commonly regulated genes, with 25 upregulated genes forming a protein-protein interaction network centered on IL1b. These genes were associated with pathways related to cytokine signaling, T cell differentiation and extracellular matrix interactions. AMLE treatment enhanced DC chemotaxis, antigen presentation and promoted T cell differentiation signaling. Specifically, flow cytometric analysis shows that AMLE increased the expression of major histocompatibility complex class II (MHCII) and the co-stimulatory molecules CD40 and CD80, leading to BMDC maturation and expansion of the CD11c+MHCIIhi and CD11c+CD11blo subsets. These findings suggest that AMLE induces potent maturation responses through multiple pathways within DC subpopulations. By elucidating the transcriptional and epigenetic effects of AMLE on BMDCs, this study contributes to a broader understanding of plant-derived compounds in immune modulation.
2025-04-30 | GSE292962 | GEO
Project description:In this study, we performed a comprehensive proteomic analysis of mango leaves inoculated with the leaf spot pathogen A. alternata. Down-regulated proteins during pathogen invasion and colonization were primarily associated with photosynthesis, the phenylpropanoid and flavonoid biosynthesis pathways, and phenylalanine metabolism. In contrast, significantly up-regulated proteins were involved in tyrosine metabolism and the MAPK signaling pathway, highlighting their critical role in host resistance to the leaf spot pathogen. These findings provide valuable data on protein expression changes, offering potential targets for developing novel management strategies to enhance control of leaf spot disease.
2025-05-07 | PXD060671 | Pride