Project description:This study utilises multiomic profiling of early-passage melanoma cell lines to explore the interactions between distinct modalities of molecular regulation influencing melanoma cellular phenotype.
Project description:RNAseq and Small-RNAseq dataset to comprehensively study the miRNA expression profiling of drug-resistant melanoma patients and cell lines
Project description:To investigate mechanisms of resistance to BRAF inhibitor therapy in melanoma, BRAF mutant cell lines have been chronically exposed to BRAFi to create phenotypes with acquired drug resistance. Activity-based protein profiling with desthiobiotinylating ATP probes (ActivX, Thermo) is used to examine the differences between naive and drug-resistant cells.
Project description:Melanoma cell lines were established and used for methylome profiling with Illumina HM450 beadchip. The molecular alterations in selected invasive melanoma cell populations were compared to those in the original cell lines by performing DNA methylation (Illumina HM450K) assays
Project description:Melanoma is a very aggressive type of skin cancer, which renders it difficult to treat because of extensive heterogeneity frequently observed in melanoma tumours. Here we hypothesized that gene expression and DNA methylation differences would correlate with invasiveness in melanoma cells. To address this question, we carried out genome-wide transcriptome and methylome investigations in non-invasive and invasive groups of melanoma cell lines.
Project description:n this project we have sequenced the transcriptome of several comonly used mouse melanoma cell lines. Our aim is to understand what mutations and rearrangements are found in these cell lines to gain a better understanding of the genes that contribute to melanomagenesis. As these cell lines are used as models of melanoma these sequences represent an important resource for functional studies. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/