Project description:The goal of this RNA-Seq analysis was to identify genes differentially expressed in wild type (N2) and a hypomorphic mutant of the gene encoding heat shock factor 1 {hsf-1(sy441)} at normal conditions and upon heat shock. This study aimed to identify genes that are up- or downregulated when HSF-1 activity is impaired at 20 ºC. We also aimed to identify gene that are regulated by HSF-1 upon heat stress by comparing differentially expressed genes upon heat shock in wild type and in hsf-1(sy441) mutant background.
Project description:Mesenchymal stromal cells (MSCs) are being tested in numerous clinical trials, yet the limited progression of these trials to advanced stages indicates unresolved translational challenges. Expanding MSCs is a critical step in most therapeutic applications and bioreactor-based culture offers large-scale production compared to monolayer cultures. Nevertheless, since MSCs sense their microenvironment, it is crucial to understand how these systems affect their properties. We expanded human adipose-derived mesenchymal stromal cells (AD-MSCs) from the same donors in both small- and large-scale platforms. Bulk-RNA sequencing and mass-spectrometry analysis demonstrate that small-scale culture had a broader range of differentially expressed genes and proteins within immunomodulatory, cell migration and cell adhesion pathways. In contrast, the large-scale culture showed a lower amount of differentially expressed genes - and proteins were proteinsparticularly associated mainly with extracellular matrix synthesis. Our findings demonstrate that expansion platforms significantly impact MSCs’ properties of MSCs, highlighting the need with optimise expansion conditions to obtain high cell yield without compromising MSCs’ their attributes.
Project description:Purpose: We used RNA-Seq to compare the gene expression profiles in two long-lived C. elegans strains: (1) animals with a loss-of-function mutation in the hsb-1 gene, and (2) animals overexpressing hsf-1 under its endogenous promoter. Previous studies indicated that life span extension in both these strains is hsf-1-dependent. Methods: mRNA-Seq profiles for three biological replicates each of day 1 adult wild-type (N2 strain), hsb-1 mutant and hsf-1 overexpression strains were generated using 50 bp single-end sequencing on an Illumina HiSeq 2500 platform. The sequencing reads that passed quality control were aligned to the C. elegans reference transcriptome (WS220 release) using Bowtie 2 and splicing junctions were mapped using TopHat. Cufflinks was used to calculate FPKM values and CuffDiff 2 was used to identify differentially expressed genes based on the following two criteria: false discovery rate (FDR)-adjusted p value < 0.05 and fold-change ≥ 1.5. Results: Using our mRNA-Seq workflow, we mapped at least 14 million reads per sample to the C. elegans transcriptome at greater than 96 percent alignment rate for the raw reads. Expression of 7,478 genes was found to be significantly altered in the hsf-1 overexpression strain relative to wild-type worms, while only 1,855 genes had singnificantly different expression in the hsb-1 mutant strain relative to wild-type. Using our criterion of ≥ 1.5-fold change in gene expression, we found 1,820 and 662 genes to be differentially expressed relative to wild-type in hsf-1 overexpression and hsb-1 mutant strains, respectively. The 247 genes that were differentially expressed in both hsf-1 overexpression and hsb-1 mutant strains showed a strongly correlated expression pattern in the two strains. Conclusions: hsf-1 overexpression induces global transcriptional suppression in C. elegans. Genetic ablation of hsb-1 produces similar life span extension in worms as hsf-1 overexpression, but alters expression of a much smaller subset of the C. elegans transcriptome. Hence, HSB-1 is a specific regulator of the transactivation potential of HSF-1 that selectively modulates gene expression relevant to longevity determination in worms.
Project description:Rat lung-resident mesenchymal stem cells (LR-MSCs) were isolated via bronchoalveolar lavage from fibroblast growth factor-10 (FGF-10) pretreated lungs. We characterized the similarity and diversity between LR-MSCs and bone marrow-derived mesenchymal stem cells (BM-MSCs) by transcriptional profiling of these two types of cells. In this dataset, we include the expression data obtained from LR-MSCs and BM-MSCs cultured under similar conditions at passage 5. These data are used to obtain genes that are differentially expressed by these two types of cells. 6 Total samples were analyzed. Both LR-MSCs and BM-MSCs are in three replicates.
Project description:Rat lung-resident mesenchymal stem cells (LR-MSCs) were isolated via bronchoalveolar lavage from fibroblast growth factor-10 (FGF-10) pretreated lungs. We characterized the similarity and diversity between LR-MSCs and bone marrow-derived mesenchymal stem cells (BM-MSCs) by transcriptional profiling of these two types of cells. In this dataset, we include the expression data obtained from LR-MSCs and BM-MSCs cultured under similar conditions at passage 5. These data are used to obtain genes that are differentially expressed by these two types of cells.
2015-04-25 | GSE68243 | GEO
Project description:Differentially expressed genes in double and single fertilized seeds
