Project description:The role of CD34 lineage cells in aortic allografts remains unintelligible. To characterize the CD34 lineage cells in aortic allografts, Cd34-CreERT2 mice were crossed with Rosa26-tdTomato mice to generate Cd34-CreERT2; Rosa26-tdTomato mice and label CD34 lineage cells in mice. We further performed single-cell RNA sequencing of whole remodeled vessel cells from Cd34-CreERT2; Rosa26-tdTomato mice with a murine model of aortic allograft (AG) at an early (AG 2w) and late stage (AG 4w). The single-cell RNA-seq analyses revealed the heterogeneity of CD34 lineage cells in vascular remodeling.
Project description:We reported the transcriptional profiling of tdTomato-labeled c-Kit lineage cells in a mouse model of transplant arteriosclerosis by single-cell RNA sequencing.
Project description:We have applied single cell RNA sequencing (scRNA-seq) as an unbiased transcriptomics to characterize aortic immune cells in atherosclerosis. The scRNA-seq analysis revealed macrophage heterogeneity at the single-cell level in mouse atherosclerotic aorta.
Project description:We generated a novel genetic mouse model of fatal aortic dissection by introducing a pathogenic variant of Fbn1 (Fbn1G234D/G234D), identified in a patient with familial aortic dissection, into mice using the CRISPR/Cas9 system. To investigate the cellular heterogeneity underlying the progression of aortic dissection, we performed single cell RNA sequencing on aortic tissues obtained from Fbn1G234D/G234D (AD) and wild-type mice (WT).