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Prokaryotic Single-Cell RNA Sequencing by In Situ Combinatorial Indexing

ABSTRACT: Prokaryotic Single-Cell RNA Sequencing by In Situ Combinatorial Indexing

PROVIDER: PRJNA591783 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10533473_1.fastq.gz	Fastqsanger.gz
	SRR10533473_2.fastq.gz	Fastqsanger.gz
	SRR10533474_1.fastq.gz	Fastqsanger.gz
	SRR10533474_2.fastq.gz	Fastqsanger.gz
	SRR10533475_1.fastq.gz	Fastqsanger.gz

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Prokaryotic Single-Cell RNA Sequencing by In Situ Combinatorial Indexing

Project description:We present Prokaryotic Expression-profiling by Tagging RNA In Situ and sequencing (PETRI-seq), a high-throughput prokaryotic scRNA-seq pipeline. We demonstrated that PETRI-seq effectively barcoded single bacterial cells in a species-mixing experiment with E. coli (MG1655) and S. aureus (USA300). Within the S. aureus population, we found rare prophage induction in 0.04% of cells. We further demonstrated that PETRI-seq was able to distinguish between E. coli growth phases based on mRNA expression patterns by combining stationary E. coli with exponential E. coli in multiple experiments.

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