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RNA sequencing of Schizosaccharomyces pombe cells with the replaced fatty acid synthase complex from Schizosaccharomyces japonicus

ABSTRACT: RNA sequencing of Schizosaccharomyces pombe cells with the replaced fatty acid synthase complex from Schizosaccharomyces japonicus

PROVIDER: PRJNA594011 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10602866.fastq.gz	Fastqsanger.gz
	SRR10602867.fastq.gz	Fastqsanger.gz
	SRR10602868.fastq.gz	Fastqsanger.gz
	SRR10602869.fastq.gz	Fastqsanger.gz

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RNA sequencing of Schizosaccharomyces pombe cells with the replaced fatty acid synthase complex from Schizosaccharomyces japonicus

Project description:We have identified that these two organisms have quite distinct phospholipid structures - S. pombe phospholipids display canonical symmetric long acyl chains (18 and/ or 16 carbon atoms), and in S. japonicus significant fraction of phospholipids are asymmetrical - one acyl chain is 18 carbon atoms and second one is short - only 10 carbon atoms. Medium chain fatty acid like C10:0 are critical for S. japonicus physiology and are synthesised by the cytosolic fatty acid synthase (FAS) complex. Such findings lead us to address the functional role of these asymmetric lipids and we exchanged FAS between the two fission yeast species. We observed that in S. pombe expressing solely japonicus FAS, the membrane is functionally impaired in a number of ways (e.g. endocytosis, mitosis etc etc) and moreover, it seems that at lower temperatures (such as 24C) the phenotype is more severe compared to the higher temperatures like 30 and 36. We believe that comparative transcriptomic analysis will shed light on what is going on in S. pombe cells with exchanged FAS and therefore displaying an altered lipidome.

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