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ABSTRACT: Transcriptional profiling of Drosophila ureter and lower tubules

PROVIDER: PRJNA595625 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10691928.fastq.gz	Fastqsanger.gz
	SRR10691929.fastq.gz	Fastqsanger.gz
	SRR10691930.fastq.gz	Fastqsanger.gz
	SRR10691931.fastq.gz	Fastqsanger.gz
	SRR10691932.fastq.gz	Fastqsanger.gz

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RNA-SEQ analysis of various developing and adult kidney components.

Project description:We used micro-dissection techniques and/or FACS to isolate cell types from the developing and adult kidney (E11.5 ureteric buds, E12.5, P1 and P4 cap mesenchyme, E15.5 collecting ducts, proximal tubules, ureter, Adult renal proximal tubules, podocytes, endothelial and mesangial cells). RNA-SEQ analysis was performed to determine the transcriptional profile of each cell type, identify component specific transcripts and isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database. Total RNA is obtained from micro-dissected and/or FACS isolated embryonic and adult kidney components. The long term goal is to generate a transcriptional atlas of developing kidney.

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Comparison of Drosophila melanogaster Malpighian tubule v whole fly

Project description:This is an affymetrix array experiment comparing the transcriptome of the Malpighian tubule (or renal tubule) of 7-day adult Oregon R strain Drosophila melanogaster with matched whole fly samples. There are five tubule samples (each derived from approx 1000 tubules (!)), and 5 matched whole-fly samples. i.e. tubule 2 is dissected from the same vial as WholeFly2. As the tubule is probably the premier tissue for true physiology in Drosophila, the dataset can usefully be interrogated in conjunction with the detailed physiological understanding of the tissue: see http://fly.to/tubules

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