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Base-resolution analysis of deoxyuridine at genome scale based on artificial incorporation modified nucleobase

ABSTRACT: Base-resolution analysis of deoxyuridine at genome scale based on artificial incorporation modified nucleobase

PROVIDER: PRJNA595860 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10700783_1.fastq.gz	Fastqsanger.gz
	SRR10700783_2.fastq.gz	Fastqsanger.gz
	SRR10700784_1.fastq.gz	Fastqsanger.gz
	SRR10700784_2.fastq.gz	Fastqsanger.gz
	SRR11968204_1.fastq.gz	Fastqsanger.gz

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Base-resolution analysis of deoxyuridine at genome scale based on artificial incorporation modified nucleobase

Project description:Deoxyuridine (dU) in DNA can result from deamination of cytosine and dUMP misincorporation. The easy single-nucleotide resolution assay for dU are crucial to understanding its role in genome. Herein, we present a new concept of “base substitution” for accurate single-nucleotide resolution profiling of dU. The method termed AI-Seq (Artificial Incorporation of a modified nucleobase for sequencing) was developed using artificial cytosine (N3-C) to replace dU. After the artificial base construction, dU can read as cytosine during polymerase chain reaction assay. AI-seq was validated on synthetic DNA and then applied to the genome of HEK293T cell line. Collectively, the “base substitution” provides a novel approach for generating comprehensive information about the distribution of dU and can be potentially adapted to detect other epigenetic modifications.

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