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Muscle injury causes gene expression changes that are related to long-term changes in stem cell DNA methylation [RNA-Seq]

ABSTRACT: Muscle injury causes gene expression changes that are related to long-term changes in stem cell DNA methylation [RNA-Seq]

PROVIDER: PRJNA595926 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10704063.fastq.gz	Fastqsanger.gz
	SRR10704064.fastq.gz	Fastqsanger.gz
	SRR10704065.fastq.gz	Fastqsanger.gz
	SRR10704066.fastq.gz	Fastqsanger.gz
	SRR10704067.fastq.gz	Fastqsanger.gz

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Muscle injury causes long-term changes in stem cell DNA methylation [RRBS]

Project description:Muscle injury causes long-term changes in stem cell DNA methylation [RRBS]

| PRJNA601988 | ENA

Muscle injury causes gene expression changes that are related to long-term changes in stem cell DNA methylation [RNA-Seq]

Project description:Injury to muscle brings about the activation of stem cells, which then generate new myocytes to replace the damaged tissue. We now demonstrate that this activation causes a dramatic change in the stem cell methylation pattern that prepares them epigenetically for terminal myocyte differentiation. These demethylation and de novo methylation events occur at regulatory elements associated with genes involved in myogenesis. Local injury of one muscle brings about an almost identical epigenetic change in satellite cells from other muscles in the body, as well. Furthermore, this same methylation state is also generated in muscle stem cells of female animals following full-term pregnancy, even in the absence of any injury. In all of these cases, which appear to be mediated by circulating factors, the new methylation profile is then stably maintained in resident muscle stem cells and thus represents a molecular memory of previous physiological events that is probably programmed to provide a mechanism for adapting to the environment. Here we show the changes in gene expression that accompany the changes in DNA methylation.

2022-12-01 | GSE142111 | GEO

Muscle injury causes long-term changes in stem cell DNA methylation [RRBS]

2022-12-01 | GSE143890 | GEO

Muscle injury causes long-term changes in stem-cell DNA methylation.

Project description:Injury to muscle brings about the activation of stem cells, which then generate new myocytes to replace damaged tissue. We demonstrate that this activation is accompanied by a dramatic change in the stem-cell methylation pattern that prepares them epigenetically for terminal myocyte differentiation. These de- and de novo methylation events occur at regulatory elements associated with genes involved in myogenesis and are necessary for activation and regeneration. Local injury of one muscle elicits an almost identical epigenetic change in satellite cells from other muscles in the body, in a process mediated by circulating factors. Furthermore, this same methylation state is also generated in muscle stem cells (MuSCs) of female animals following pregnancy, even in the absence of any injury. Unlike the activation-induced expression changes, which are transient, the induced methylation profile is stably maintained in resident MuSCs and thus represents a molecular memory of previous physiological events that is probably programmed to provide a mechanism for long-term adaptation.

| S-EPMC9907067 | biostudies-literature

Muscle injury prepares stem cells for terminal myocyte differentiation through changes in gene expression and long-term changes in DNA methylation

Project description:Muscle injury prepares stem cells for terminal myocyte differentiation through changes in gene expression and long-term changes in DNA methylation

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Changes in Protein Fluxes and Gene Expression in Non-Injured Muscle Tissue Distant from an Acute Myotoxic Injury in Male Mice

Project description:The response to acute myotoxic injury requires stimulation of local repair mechanisms in the damaged tissue. However, satellite cells in muscle distant from acute injury have been reported to enter a functional state between quiescence and active proliferation. Here, we asked whether protein flux rates are altered in muscle distant from acute local myotoxic injury and how they compare to changes in gene expression from the same tissue. Broad and significant alterations in protein turnover were observed across the proteome in the limb contralateral to injury during the first 10 days after. Interestingly, mRNA changes had almost no correlation with directly measured protein turnover rates. In summary, we show consistent and striking changes in protein flux rates in muscle tissue contralateral to myotoxic injury, with no correlation between changes in mRNA levels and protein synthesis rates. This work motivates further investigation of the mechanisms, including potential neurologic factors, responsible for this distant effect.

2024-04-30 | PXD051860 | JPOST Repository

Hypercapnia induces long-term DNA methylation changes in skeletal muscle.

Project description:Hypercapnia induces long-term DNA methylation changes in skeletal muscle.

| PRJNA1263460 | ENA

Hypercapnia induces long-term DNA methylation changes in skeletal muscle

Project description:Hypercapnia induces long-term DNA methylation changes in skeletal muscle

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Microglia adopt longitudinal transcriptional changes after traumatic brain injury

Project description:Traumatic brain injury (TBI) is an under-recognizedpublic healththreat. Even mild brain injuries can lead to long-term neurologic impairment.Microgliaplay a fundamental role in the development and progression of this ensuing neurologic impairment. Despite this, a microglia-specific injury signature has yet to be identified. We hypothesized that TBI would lead to long-term changes in the transcriptional profile of microglial pathways associated with the development of subsequent neurologic impairment.

2023-12-11 | GSE249918 | GEO

Myeloid C3 causes neuronal injury prior to synapse loss

Project description:Myeloid C3 causes neuronal injury prior to synapse loss

| PRJNA1084770 | ENA