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ABSTRACT: Leaf transcriptome of transgenic Nicotiana tabacum overexpressing LcSABP under hydroponic conditions with triclosan treatment

PROVIDER: PRJNA596122 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10716746_1.fastq.gz	Fastqsanger.gz
	SRR10716746_2.fastq.gz	Fastqsanger.gz
	SRR10716747_1.fastq.gz	Fastqsanger.gz
	SRR10716747_2.fastq.gz	Fastqsanger.gz
	SRR10716748_1.fastq.gz	Fastqsanger.gz

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Overexpression of NtmybA2 in tobacco BY-2 cells

Project description:Promoters of many G2/M phase-specific genes in plants contain mitosis-specific activator (MSA) elements, which act as G2/M phase-specific enhancers and bind with R1R2R3-Myb transcription factors. A least three R1R2R3-Myb proteins are expressed in tobacco (Nicotiana tabacum). To examine the genome-wide effects of NtmybA2 overexpression, we constructed transgenic tobacco BY-2 cell lines overexpressing full-length NtmybA2 protein. In addition, we generated transgenic BY-2 cell lines that overexpress truncated hyperactive form of NtmybA2 (NtmybA2âC) lacking its C-terminal region which negatively regulates its own activity for transcriptional activation. We used a custom-made 16K cDNA microarray for comparative transcriptome analysis of these transgenic tobacco BY-2 cell lines. As control sample, BY-2 cells transformed with empty vector pPZP-35S were used. The transgenic cell lines were analyzed during stationary phase (i.e. day 7-8 after subculture into fresh media). Keywords: Genetic modification 1. pPZP-35S vector control, 2. NtmybA2 overexpressor, 3. C-truncated NtmybA2 (NtmyA2âC) overexpressor

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