Project description:Biochar is widely used in water remediation, but its application risks in water bodies have not yet been revealed. This project aims to reveal the potential of biochar in water remediation and its related environmental risks.
2024-12-31 | PXD059333 |
Project description:Biochar facilitating electron transfer of anaerobic granular sludge
Project description:Microbial extracellular electron uptake (EEU) is central to bioelectrochemical processes and biocorrosion, yet its molecular mechanisms remain incompletely understood. Here, we investigate how excess Fe2+ modulates EEU in Desulfovibrio ferrophilus IS5, a strain that causes severe anaerobic iron corrosion via outer-membrane cytochromes (OMCs)-mediated electron uptake. We show that IS5 grown with elevated Fe2+ exhibits substantially enhanced EEU. This enhancement arises through two complementary mechanisms: (i) increased abundance of functional OMCs via upregulation of a cytochrome assembly protein, and (ii) an additional electron transfer route mediated by FeS nanoparticles precipitated on the IS5 outer membrane. Remarkably, IS5 with low OMCs expression but biosynthesized FeS can rapidly shift to EEU before OMCs induction. These findings suggest that during iron corrosion, when IS5 cells are embedded within thick corrosion crusts and biofilms and face both high Fe2+ concentrations and organic limitation, they exploit OMCs and FeS nanoparticles in parallel to sustain high-rate EEU from iron. This study advances the mechanistic understanding of EEU-driven iron corrosion and highlights a potential avenue for manipulating bioelectrochemical systems.
Project description:Geobacteraceae transfer electrons from a donor such as acetate to an electron acceptor such as Fe(III) or U(VI). Geobacter uraniireducens is found in uranium-contaminated sites and plays an important role in in situ bioremediation. In this experiment, gene expression was compared between G. uraniireducens cultures grown in sediments from a uranium contaminated site amended with acetate and cultures grown in acetate/fumarate medium. Keywords: two-condition comparison
2008-10-20 | GSE10920 | GEO
Project description:Alfalfa-biochar-microbial combined remediation of MTBE-contaminated soil
| PRJNA1280981 | ENA
Project description:Ryegrass-biochar-microbial combined remediation of MTBE-contaminated soil
| PRJNA1280939 | ENA
Project description:Biochar facilitating electron transfer of anaerobic granular sludge-archaea
| PRJNA784693 | ENA
Project description:biochar immobilized microbes to remediate contaminated soils