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Physical interactions between Gsx2 and Ascl1 balance progenitor expansion versus neurogenesis in the mouse lateral ganglionic eminence

ABSTRACT: Physical interactions between Gsx2 and Ascl1 balance progenitor expansion versus neurogenesis in the mouse lateral ganglionic eminence

PROVIDER: PRJNA598224 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10806517_1.fastq.gz	Fastqsanger.gz
	SRR10806517_2.fastq.gz	Fastqsanger.gz
	SRR10806518_1.fastq.gz	Fastqsanger.gz
	SRR10806518_2.fastq.gz	Fastqsanger.gz
	SRR10806519_1.fastq.gz	Fastqsanger.gz

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Physical interactions between Gsx2 and Ascl1 balance progenitor expansion versus neurogenesis in the mouse lateral ganglionic eminence

Project description:These findings suggest that physical interactions between Gsx2 and Ascl1 limit Ascl1:Ascl1 and Ascl1:Tcf3 interactions, and thereby inhibit Ascl1-dependennt neurogenesis and allow for progenitor expansion within the LGE.

2020-03-11 | GSE142768 | GEO

Physical interactions between Gsx2 and Ascl1 balance progenitor expansion versus neurogenesis in the mouse lateral ganglionic eminence.

Project description:The Gsx2 homeodomain transcription factor promotes neural progenitor identity in the lateral ganglionic eminence (LGE), despite upregulating the neurogenic factor Ascl1. How this balance in maturation is maintained is unclear. Here, we show that Gsx2 and Ascl1 are co-expressed in subapical progenitors that have unique transcriptional signatures in LGE ventricular zone (VZ) cells. Moreover, whereas Ascl1 misexpression promotes neurogenesis in dorsal telencephalic progenitors, the co-expression of Gsx2 with Ascl1 inhibits neurogenesis. Using luciferase assays, we found that Gsx2 reduces the ability of Ascl1 to activate gene expression in a dose-dependent and DNA binding-independent manner. Furthermore, Gsx2 physically interacts with the basic helix-loop-helix (bHLH) domain of Ascl1, and DNA-binding assays demonstrated that this interaction interferes with the ability of Ascl1 to bind DNA. Finally, we modified a proximity ligation assay for tissue sections and found that Ascl1-Gsx2 interactions are enriched within LGE VZ progenitors, whereas Ascl1-Tcf3 (E-protein) interactions predominate in the subventricular zone. Thus, Gsx2 contributes to the balance between progenitor maintenance and neurogenesis by physically interacting with Ascl1, interfering with its DNA binding and limiting neurogenesis within LGE progenitors.

| S-EPMC7157596 | biostudies-literature

Bulk RNA-seq of the human fetal neocortex, lateral ganglionic eminence and medial ganglionic eminence at 7, 9, 11 and 20 post-conceptional weeks

Project description:Deep transcriptional profiling of the human neocortex, lateral ganglionic eminence (LGE) and medial ganglionic eminences (MGE), from 7 to 20 post-conceptional weeks (pcw), for de novo lincRNAs discovery and to establish a unique coding and non-coding gene signature for the three different regions.

2021-05-13 | E-MTAB-8893 | biostudies-arrayexpress

Transcriptomic analysis from lateral ganglionic eminence (LGE) of wild type and Gsx2 germline knock-out mice.

Project description:Transcriptomic analysis from lateral ganglionic eminence (LGE) of wild type and Gsx2 germline knock-out mice.

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Conserved Gsx2/Ind homeodomain monomer versus homodimer DNA binding defines regulatory outcomes in the fly and mouse

Project description:Identification of Gsx2 genomic binding patterns from E12.5 mouse lateral ganglionic eminence (LGEs).

2020-12-04 | GSE162589 | GEO

Transcriptomic analysis from lateral ganglionic eminence (LGE) of wild type and Gsx2 germline knock-out mice.

Project description:RNA-seq analysis was used to identify genes that are differentially expressed between wild type and Gsx2 null mouse LGEs.

2020-12-04 | GSE162590 | GEO

Comparative expression of embryonic medial- and lateral/caudal-ganglionic eminence cells migrating in the mouse cerebral cortex

Project description:Cortical interneurons originate in the medial and caudal ganglionic eminence and migrate into the cortex during embryogenesis. We purified cells migrating within the cortex at different embryonic stages and compared their transcriptome to identify transcriptional programmes underlying distinct cortical interneuron fates.

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CTCF occupancy in the mouse telencephalon and medial ganglionic eminence

Project description:In this study, we wanted to compare CTCF binding sites in whole telencephalon vs medial ganglionic eminence (MGE) at embryonic day E13.5.

2019-04-05 | GSE105071 | GEO

Single-cell RNA-sequencing data of human medial ganglionic eminence organoids

Project description:We analysed scRNA-seq data in human pluripotent stem cell-derived medial ganglionic eminence (MGE) organoids. This in vitro system recapitulates some key aspects of humam MGE development including inhibitory cortical interneuron diversification.

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