Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mechanism of selective incorporation of lncRNA into a chromatin modifier

ABSTRACT: Mechanism of selective incorporation of lncRNA into a chromatin modifier

PROVIDER: PRJNA600524 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10871011_1.fastq.gz	Fastqsanger.gz
	SRR10871011_2.fastq.gz	Fastqsanger.gz
	SRR10871012_1.fastq.gz	Fastqsanger.gz
	SRR10871012_2.fastq.gz	Fastqsanger.gz
	SRR10871013_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 142

Similar Datasets

Mechanism of selective incorporation of lncRNA into a chromatin modifier

Project description:The MLE DExH helicase and the roX lncRNAs are essential components of the chromatin modifying Dosage Compensation Complex (DCC) in Drosophila. To explore the mechanism of ribonucleoprotein complex assembly, we designed vitRIP, an unbiased, transcriptome-wide in vitro assay that reveals RNA binding specificity. We found that MLE has intrinsic specificity for U-rich sequences and tandem stem-loop structures. In vitro, the helicase binds and remodels many RNAs beyond its main target, roX2. Unwinding of roX2 by the helicase triggers their selective association with the DCC, via the MSL2 subunit. Whereas the core DCC alone does not show intrinsic RNA binding specificity, the presentation of remodeled roX2 by MLE induces a highly selective RNA binding surface in the unstructured C-terminus of MSL2. The exquisite selectivity of roX2 incorporation into the DCC thus originates from intimate cooperation between the helicase and the core DCC involving two distinct RNA selection principles and their mutual refinement.

2020-05-27 | GSE143455 | GEO

Two-step mechanism for selective incorporation of lncRNA into a chromatin modifier.

Project description:The MLE DExH helicase and the roX lncRNAs are essential components of the chromatin modifying Dosage Compensation Complex (DCC) in Drosophila. To explore the mechanism of ribonucleoprotein complex assembly, we developed vitRIP, an unbiased, transcriptome-wide in vitro assay that reveals RNA binding specificity. We found that MLE has intrinsic specificity for U-/A-rich sequences and tandem stem-loop structures and binds many RNAs beyond roX in vitro. The selectivity of the helicase for physiological substrates is further enhanced by the core DCC. Unwinding of roX2 by MLE induces a highly selective RNA binding surface in the unstructured C-terminus of the MSL2 subunit and triggers-specific association of MLE and roX2 with the core DCC. The exquisite selectivity of roX2 incorporation into the DCC thus originates from intimate cooperation between the helicase and the core DCC involving two distinct RNA selection principles and their mutual refinement.

| S-EPMC7528653 | biostudies-literature

Rare codon recoding for selective and efficient noncanonical amino acid incorporation [Ribo-seq]

Project description:The ability to genetically encode noncanonical amino acids (ncAAs) has empowered proteins with improved or novel properties. However, existing strategies in mammalian cells rely on the introduction of blank codon for incorporating ncAAs, which is very inefficient and limits their widespread applications. Here, we develop a rare codon recoding strategy that takes advantage of the relative rarity of the TCG codon to achieve highly selective and efficient ncAA incorporation through systematic engineering and big data model predictions. We highlight the broad utility of this strategy for the incorporation of dozens of ncAAs into various functional proteins at the wild-type protein expression levels, as well as the synthesis of proteins with up to 6-site ncAAs or 4 distinct ncAAs in mammalian cells for downstream applications.

2025-01-01 | GSE255465 | GEO

Rare codon recoding for selective and efficient noncanonical amino acid incorporation [RNA-seq]

2025-01-01 | GSE255464 | GEO

Rare codon recoding for selective and efficient noncanonical amino acid incorporation [Ribo-seq]

Project description:Rare codon recoding for selective and efficient noncanonical amino acid incorporation [Ribo-seq]

| PRJNA1075071 | ENA

Rare codon recoding for selective and efficient noncanonical amino acid incorporation [RNA-seq]

Project description:Rare codon recoding for selective and efficient noncanonical amino acid incorporation [RNA-seq]

| PRJNA1075072 | ENA