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Aging induces aberrant state transition kinetics in murine muscle stem cells

ABSTRACT: Aging induces aberrant state transition kinetics in murine muscle stem cells

PROVIDER: PRJNA600730 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10874104.fastq.gz	Fastqsanger.gz
	SRR10874105.fastq.gz	Fastqsanger.gz
	SRR10874106.fastq.gz	Fastqsanger.gz
	SRR10874107.fastq.gz	Fastqsanger.gz
	SRR10874108.fastq.gz	Fastqsanger.gz

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Aging induces aberrant state transition kinetics in murine muscle stem cells.

Project description:Murine muscle stem cells (MuSCs) experience a transition from quiescence to activation that is required for regeneration, but it remains unknown if the trajectory and dynamics of activation change with age. Here, we use time-lapse imaging and single cell RNA-seq to measure activation trajectories and rates in young and aged MuSCs. We find that the activation trajectory is conserved in aged cells, and we develop effective machine-learning classifiers for cell age. Using cell-behavior analysis and RNA velocity, we find that activation kinetics are delayed in aged MuSCs, suggesting that changes in stem cell dynamics may contribute to impaired stem cell function with age. Intriguingly, we also find that stem cell activation appears to be a random walk-like process, with frequent reversals, rather than a continuous linear progression. These results support a view of the aged stem cell phenotype as a combination of differences in the location of stable cell states and differences in transition rates between them.

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Aging induces aberrant state transition kinetics in murine muscle stem cells

Project description:Label retaining and non-retaining muscle stem cells from young and aged H2B-GFP+/-;rtTA+/- were profiled by single cell RNA-seq at two timepoints

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Granulins Regulate Aging Kinetics in the Adult Zebrafish Telencephalon

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Granulins Regulate Aging Kinetics in the Adult Zebrafish Telencephalon

Project description:Granulins (GRN) are secreted factors that promote neuronal survival and regulate inflammation in various pathological conditions. However, their roles in physiological conditions in the brain remain poorly understood. To address this knowledge gap, we analysed the telencephalon in Grn-deficient zebrafish and identified morphological and transcriptional changes in microglial cells, indicative of a pro-inflammatory phenotype in the absence of any insult. Unexpectedly, activated mutant microglia shared part of their transcriptional signature with aged human microglia. Furthermore, transcriptome profiles of the entire telencephali isolated from young Grn-deficient animals showed remarkable similarities with the profiles of the telencephali isolated from aged wildtype animals. Additionally, 50% of differentially regulated genes during aging were regulated in the telencephalon of young Grn-deficient animals compared to their wildtype littermates. Importantly, the telencephalon transcriptome in young Grn-deficent animals changed only mildly with aging, further suggesting premature aging of Grn-deficient brain. Indeed, Grn loss led to decreased neurogenesis and oligodendrogenesis, and to shortening of telomeres at young ages, to an extent comparable to that observed during aging. Altogether, our data demonstrate a role of Grn in regulating aging kinetics in the zebrafish telencephalon, thus providing a valuable tool for the development of new therapeutic approaches to treat age-associated pathologies.

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Aberrant engagement of P-selectin drives hematopoietic stem cell aging

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Aberrant overexpression of TGF-β2 induces epithelial-mesenchymal transition in adenomyosis with β-catenin activation.

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De novo DNA methylation suppresses aberrant fate trajectory during epiblast transition [ATAC-seq]

Project description:De novo DNA methylation suppresses aberrant fate trajectory during epiblast transition [ATAC-seq]

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De novo DNA methylation suppresses aberrant fate trajectory during epiblast transition [RNA-seq]

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De novo DNA methylation suppresses aberrant fate trajectory during epiblast transition [RNA-seq]

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