Project description:Characterization of Early Bacterial and Fungal Communities on Volcanic Deposits along a Vegetation Gradient in the Island of Miyake, Japan
Project description:To characterize basal differences in hepatic gene-expression patterns, we performed a microarray analysis using wild-type and L1-Tg mice fed a control fat diet (CE-2: CLEA Japan, Inc., Tokyo, Japan) for two weeks.
Project description:UPLC-MS/MS or nanoLC-MS/MS data acquired from whole sponge extracts from the marine sponge Theonella swinhoei (yellow chemotype; Hachijo Jima, Japan) or extracts from two Entotheonella enriched cell fractions obtained by differential centrifugation. More than 40 bioactive polyketides and modified peptides that belong to seven structural classes are known to be produced by this sponge. Single-cell genomics and chemical analysis have revealed Entotheonella as the producer of most of the known chemistry from this sponge.
Project description:Copy number analysis of primary esophageal squamous cell carcinoma (ESCC) from 40 patients in Japan. Integrative analysis of gene expression profiles and genomic alterations obtained from array-CGH and NGS provided us new insight into the pathogenesis of ESCC.
Project description:Lions and tigers, as dominant apex predators, likely became competitors when lions expanded from Africa into Eurasia approximately one million years ago (Ma), forming a lion–tiger transition belt from the Middle East through Central Asia to the Russian Far East. At the easternmost edge of this zone, the Japanese Archipelago has long been considered a Late Pleistocene tiger refugium, supported by large felid subfossils traditionally attributed to tigers (Panthera tigris), though their taxonomic identity remained unresolved. To clarify the origin, evolutionary history, and biogeography of Japan’s Pleistocene felids, we analyzed 26 ancient specimens previously assumed to be tigers. Using mitochondrial and nuclear genome hybridization capture and sequencing, paleoproteomics, Bayesian molecular dating, and radiocarbon dating, we found that all ancient Japanese “tiger” remains yielding molecular data were, unexpectedly, cave lions (Panthera spelaea). One specimen was radiocarbon dated to 31,060 ± 190 BP. These cave lions likely dispersed to the Japanese Archipelago between ~72.7 and 37.5 thousand years ago (ka), when a land bridge connected northern Japan to the mainland during the Last Glacial Period. Our findings challenge the long-held view that tigers once took refuge in Japan, showing instead that cave lions were widespread in northeast Asia during this period and were the Panthera lineage that colonized Japan, reaching even its southwestern regions despite habitats previously thought to favor tigers.
Project description:purified standards of onnamide A, onnamide B and cyclotheonamide isolated from Theonella swinhoei (yellow chemotype, Hachijo Jima, Japan).
Reference: Wilson MC, Mori T, R�ckert C, Uria AR, Helf MJ, Takada K, Gernert C, Steffens UA, Heycke N, Schmitt S, Rinke C, Helfrich EJ, Brachmann AO, Gurgui C, Wakimoto T, Kracht M, Cr�semann M, Hentschel U, Abe I, Matsunaga S, Kalinowski J, Takeyama H, Piel J. An environmental bacterial taxon with a large and distinct metabolic repertoire. Nature. 2014 Feb 6;506(7486):58-62.
