Project description:qPCR profiling of miRNAs in mouse fetal lungs was done. Lungs were dissected and RNA was extracted using miRVANA extraction kit (Applied Biosystems).
Project description:Osteosarcoma (OS) is the primary bone tumor in children and young adults. Currently, there are no reliable, non-invasive biological markers to detect the presence or progression of disease, assess therapy response, or provide upfront prognostic insights. Using a qPCR-based platform that analyzes more than 750 miRNAs, we analyzed control and diseased-associated plasma from a genetically engineered mouse model of OS to identify a profile of four plasma miRNAs.
Project description:Osteosarcoma (OS) is the primary bone tumor in children and young adults. Currently, there are no reliable, non-invasive biological markers to detect the presence or progression of disease, assess therapy response, or provide upfront prognostic insights. Using a qPCR-based platform that analyzes more than 750 miRNAs, we analyzed control and diseased-associated plasma from a genetically engineered mouse model of OS to identify a profile of four plasma miRNAs. Plasma from mice with OS were profiled for miRNAs and compared with the profile of plasma from disease-free mice
Project description:qPCR profiling of miRNAs in mouse fetal lungs was done. Lungs were dissected and RNA was extracted using miRVANA extraction kit (Applied Biosystems). RNA was isolated from 2 separate lungs (labeled as A or B) for each time point and 2 technical replicates were run for each sample (labeled as 1 or 2).