Project description:To determine the distribution of centromere units in the genome of holocentric Chionographis japonica, we performed CENH3-ChIPseq using the customized species-specific CENH3 antibody. We mixed the chromatins of C. japonica and Secale cereal (inbred line Lo7) to dilute the highly abundant centromeric Chio satellite repeats (16%) in the C. japonica genome before immunoprecipitation. In addition, to determine the large-scale genome organization, we performed ChIPseq by targeting the evolutionarily conserved eu- and heterochromatin-specific histone marks H3K4me2 and H33K9me2

2023-05-08 | GSE228407 | GEO

Chronic clinical hepatic schistosomiasis japonica

Project description:We have performed a genome wide transcriptional survey of liver biopsies obtained from patients in Hunan China, who have chronic schistosomiasis with and without past viral hepatitis history, compared to patients with no liver disease history or indicators. These results present a comprehensive transcriptional profile of chronic schistosomiasis japonica patients and demonstrate similarities and differences with other hepatic diseases. These unique features of gene expression, in conjunction with previous reports of the cellular composition of granuloma formation and recovery, present an improved understanding of the molecular immunopathology and general physiological status underlying hepatic schistosomiasis. The gene expression profile of the human liver was examined for chronic patients with Schistosoma japonicum infections. Microarray analysis was performed on cRNA synthesised from total RNA derived from the liver biopsy tissue from huan China. Three groups were examined based on infections status [C] were without history or indicators of schistosomiasis or viral heapatitis controls ; [S] with a history or active for schistosomiasis but no indicators of viral heapatitis; and [S]

2014-09-13 | E-GEOD-61376 | biostudies-arrayexpress

Rice japonica gene expression array across eleven tissues

Project description:To validate transcription of the japonica non-exonic TARs and to understand their transcriptional relation with annotated genes, we constructed a new array (designated the re-array) to surrogate 44,385 non-TE gene models and 25,313 TARs in japonica each with five independent 36mer probes. Using the re-array, we obtained triplicate expression estimates from 11 rice tissue. Keywords: gene expression

2007-02-06 | GSE6921 | GEO

single cell RNA-Seq of Dugesia japonica

Project description:A single cell RNA-Seq analysis of Dugesia japonica intact animals by TAS-Seq (Shichino et al, 2022)

2023-09-30 | GSE223927 | GEO