Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sp8 regulatory function in the limb bud ectoderm

ABSTRACT: Sp8 regulatory function in the limb bud ectoderm

PROVIDER: PRJNA604730 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11015200.fastq.gz	Fastqsanger.gz
	SRR11015201.fastq.gz	Fastqsanger.gz
	SRR11015202.fastq.gz	Fastqsanger.gz
	SRR11015074_1.fastq.gz	Fastqsanger.gz
	SRR11015074_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Sp8 regulatory function in the limb bud ectoderm [RNA-seq]

Project description:Sp8 and Sp6, members of the Sp family of transcription factors, are required in a dose-dependent manner to induce Fgf8 and En1 in the limb bud ectoderm, therefore controlling proximo-distal and dorso-ventral limb development. Mouse genetics revealed that Sp8 makes a much greater contribution than Sp6 but the nature of its regulatory mechanism was unknown. Here, by combining ChIP-seq and RNA-seq genome-wide analyses we show that Sp8 predominantly functions as an activator from putative distal enhancers regulating crucial limb patterning genes and underscoring its master role in limb development. We also provide compelling evidence for Sp8 cooperating with Dlx5 for the regulation of a considerable set of its target genes. Our work supports a model in which Sp8, Sp6 and Dlx5 act conjointly to regulate target genes with a final functional outcome that depends on their relative availability. This should be considered when interpreting Sp and Dlx mutant phenotypes.

2021-02-01 | GSE144732 | GEO

Sp8 regulatory function in the limb bud ectoderm [ChIP-seq]

2021-02-01 | GSE144731 | GEO

Sp8 regulatory function in the limb bud ectoderm [RNA-seq]

Project description:Sp8 regulatory function in the limb bud ectoderm [RNA-seq]

| PRJNA604733 | ENA

Sp8 regulatory function in the limb bud ectoderm [ChIP-seq]

Project description:Sp8 regulatory function in the limb bud ectoderm [ChIP-seq]

| PRJNA604734 | ENA

Sp8 regulatory function in the limb bud ectoderm

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-02-01 | GSE144733 | GEO

Roles of Mettl14 in mouse limb bud development

Project description:m6A regulates virtually every step in RNA metabolism. However, its toles in limb development remains largely unknown. To understand the roles, we created a limb bud-specific conditional knockout (cKO) mice and control heterozygous (cHet) mice of the Mettl14 gene, which encodes an essential subunit in the m6A methyltransferase complex METTL3/METTL14. We harvested limb buds from the mice on E12.5 and applied the proteins to quantitative mass spectrometry to understand how the depletion of Mettl14 affected the proteomes.

2025-04-03 | PXD049092 | Pride

Bhlha9 deficiency effect in limb bud

Project description:Analysis of mouse limb bud (E10.5) lacking the Bhlha9 gene. Bhlha9 knockout mouse shows syndactyly and poliosis in the limb. This microarray results provides insight into the molecular mechanisms underlying Bhlha9 function in the limb development DNA microarray analysis was performed using Affymetrix mouse genome 430 2.0 array. RNA samples were obtained from the whole limb bud of the E10.5 wild-type and Bhlha9 knockout embryos described above. Total RNA (200 ng) was reverse-transcribed and biotinylated using the GeneChip 3â² IVT Express Kit (Affymetrix). The microarray data were summarized using the MAS 5.0 method.

2016-05-31 | E-GEOD-81223 | biostudies-arrayexpress

Mouse embryo limb bud regeneration

Project description:Mouse embryo limb bud regeneration

| PRJNA1241699 | ENA

Bhlha9 deficiency effect in limb bud

2016-05-31 | GSE81223 | GEO

Transcription profiling of mouse E10.5 limb bud ZPA and the rest of the E10.5 limb bud from Shhgfpcre heterozygotes separated by FACS

Project description:Comparing gene expression of cells from the E10.5 limb bud ZPA and the rest of the E10.5 limb bud from Shhgfpcre heterozygotes separated by FACS. Experiment Overall Design: 8 samples, 4 ZPA and 4 rest of the limb

2008-06-15 | E-GEOD-7598 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data