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Expression data from microglial cell line BV-2

ABSTRACT: Expression data from microglial cell line BV-2

PROVIDER: PRJNA606033 | ENA |

REPOSITORIES: ENA

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Expression data from microglial cell line BV-2

Project description:Microglial morphology is tightly associated with aspects of their functions during the postnatal stage. It is affected by not only intrinsic cues but also external factors. To pursue the novel factor that controls microglial morphology, we conducted microarray analysis using microglial cell line BV-2. We identified distinct candidates of both up-regulated and down-regulated genes from this experiment.

2020-02-12 | GSE145098 | GEO

Genome-wide identification and analysis of gene expression in BV-2, a murine microglial cell line, infected with rabies virus CVS-11 (Challenge virus standard)

Project description:The hypothesis tested in the present study was that rabies virus (RABV) infection affects the gene expression in microglial cells. Results provide important information that RABV infection led to alteration of gene expression in microglia. A twelve-chip study was performed using total RNA isolated from RABV- or mock-infected BV-2 at 12, 24, or 48 hpi.

2014-10-10 | E-GEOD-35684 | biostudies-arrayexpress

Transcriptome analysis of BV-2 microglial cells displaying a CRISPR/Cas9-induced knockout of peroxisomal genes (Abcd1, Abcd2, Abcd1/Abcd2, Acox1)

Project description:Here, we report the characterization and the comparison of the transcriptomes of BV-2 murine microglial mutant cell lines (CRISPR/Cas9-edited mutations in peroxisomal genes) by RNA-sequencing. Microglia is suspected to play a major role in the neurodegenerative processes observed in peroxisomal leukodystrophies. From CRISPR/Cas9-edited BV-2 microglial cell lines, we aimed at exploring the transcriptomic consequences of a defect of peroxisomal beta-oxidation.

2023-03-28 | GSE200022 | GEO

Transcriptome analysis of the LPS response of BV-2 microglial cells displaying a CRISPR/Cas9-induced knockout of peroxisomal genes (Abcd1/Abcd2 or Acox1)

Project description:In X-linked adrenoleukodystrophy (X-ALD), the most common peroxisomal disorder, microglial defect is suggested to prime and amplify the neuroinflammatory process. By using CRISPR/Cas9 gene editing, we recently established BV-2 microglial cell models to study the impact of dysfunctional peroxisomal b-oxidation and demonstrated the emergence of a disease-associated microglial signature in these cell lines. Their transcriptomic analysis suggested consequences on immune response. To go further, we have used RNA-sequencing and functional assays related to immune response to compare the WT and mutant BV-2 cell lines in basal conditions or upon lipopolysaccharide (LPS) stimulation.

2024-01-01 | GSE237635 | GEO

Docosahexaenoic acid attenuates metabolic dysfunctions induced by lipopolysaccharide in BV-2 microglial cells

Project description:Microglial cell activation has been linked to many neurodegenerative diseases. Upon stimulation by lipopolysaccharide (LPS), a number of proteins involved in inflammatory and oxidative pathways are activated. Production of nitric oxide has been regarded as a signature marker of inflammatory responses. Our recent studies demonstrated the effects of docosahexaenoic acid (DHA) to inhibit the LPS-induced inflammatory responses in BV-2 microglial cells. DHA also can upregulate the anti-oxidative pathway involving nuclear factor erythroid 2-Like 2 (Nrf2) and synthesis of heme oxygenase-1 (HO-1), a potent anti-oxidative enzyme. In order to further understand the proteins involved, this study used a label-free quantitative proteomics approach to examine effects of DHA and LPS on proteins and signaling pathways in microglial cells.

2020-04-20 | PXD013086 | Pride

Bioinformatic analysis identifies RGS10 as a modulator of BV-2 cell migration

Project description:Regulator of G protein signaling 10 (RGS10) is a GTPase activating protein, selective for Gαi, that has been proposed to play a role in suppressing microglial-driven neuroinflammation. RGS10 expression in microglia is suppressed by inflammatory stimuli and aging, and loss of RGS10 is associated with increased cytokine expression and neurodegeneration. Conversely, RGS10 overexpression provides protection against inflammatory stimuli in rodent models, however the mechanisms by which RGS10 exerts this protective effect are unknown. To understand the neuroprotective functions of RGS10 in microglia, we completed RNA-Seq analysis in the murine microglial cell line BV-2 with intact (BV-2WT) and absent (RGS10-/-) RGS10 expression under basal and IFNγ-treated conditions. RGS10 loss altered expression of 1,579 genes in unstimulated cells and 2,142 genes under IFNγ-stimulated conditions. In addition to changes in GPCR signaling, altered processes in RGS10-/- cells, under basal and stimulated conditions, included cell migration, adhesion, cytokine production and cell development. Key cell adhesion genes such as Mmp9 and Thbs1 were downregulated in RGS10-/- cells regardless of stimuli. Other genes such as Icam1 and Il1b displayed significantly lower expression only in IFNγ-stimulated RGS10-/- cells compared to their WT counterpart. Loss of RGS10 increased BV-2 cell migration, and IFNγ stimulation led to a reduction in migration of both BV-2WT and RGS10-/- cells. The effect of RGS10 on migration could only partially be blocked by the Gαi inhibitor Pertussis toxin (PTX), suggesting involvement of both G protein-dependent and -independent mechanisms. Altogether, these results suggest a novel role for RGS10 in reducing microglial migration through regulation of cell adhesion genes.

2025-07-08 | GSE301244 | GEO

Microarray and Pathway Analysis Reveal Distinct Mechanisms Underlying Cannabinoid-Mediated Modulation of LPS-Induced Activation of BV-2 Microglial Cells

Project description:Cannabinoids are known to exert immunosuppressive activities. However, the mechanisms which contribute to these effects are unknown. Using lipopolysaccharide (LPS) to activate BV-2 microglial cells, we examined how Δ9-tetrahydrocannabinol (THC), the major psychoactive component of marijuana, and cannabidiol (CBD) the non-psychoactive component, modulate the inflammatory response. Microarray analysis of genome-wide mRNA levels was performed using Illumina platform and the resulting expression patterns analyzed using the Ingenuity Pathway Analysis to identify functional subsets of genes, and the Ingenuity System Database to denote the gene networks regulated by CBD and THC.

2015-07-09 | E-GEOD-70689 | biostudies-arrayexpress

Microarray and Pathway Analysis Reveal Distinct Mechanisms Underlying Cannabinoid-Mediated Modulation of LPS-Induced Activation of BV-2 Microglial Cells

2015-07-09 | GSE70689 | GEO

RNA Sequencing Facilitates Quantitative Analysis of BV-2 Transcriptomes

Project description:Using RNA-seq, we report here that BV-2 microglial cells have a distinct transcriptomic signature and express a unique cluster of transcripts in response to 4 hrs LPS.

2016-12-04 | GSE75714 | GEO

Genome-wide expression analysis of memory-like inflammatory responses in murine micoglial cells (BV-2 immortalized microglial cell line)

Project description:Genome-wide expression analysis of memory-like inflammatory responses in murine micoglial cells (BV-2 immortalized microglial cell line)

| PRJNA566425 | ENA

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