Low-cost high-resolution chromatin profiling with CUT&Tag
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA606273 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR11074228_1.fastq.gz | Fastqsanger.gz | |||
| SRR11074228_2.fastq.gz | Fastqsanger.gz | |||
| SRR11074229_1.fastq.gz | Fastqsanger.gz | |||
| SRR11074229_2.fastq.gz | Fastqsanger.gz | |||
| SRR11074230_1.fastq.gz | Fastqsanger.gz |
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Project description:We recently introduced CUT&Tag, an epigenomic profiling strategy in which antibodies are bound to chromatin proteins in situ in permeabilized nuclei, and then used to tether the cut-and-paste transposase Tn5. Activation of the transposase simultaneously cleaves DNA and adds DNA sequencing adapters (“tagmentation”) for paired-end DNA sequencing. Here, we introduce a streamlined CUT&Tag protocol that suppresses exposure artifacts to ensure high-fidelity mapping of the antibody-targeted protein and improves signal-to-noise over current chromatin profiling methods. Streamlined CUT&Tag can be performed in a single PCR tube from cells to amplified libraries, providing low-cost high-resolution genome-wide chromatin maps. By simplifying library preparation, CUT&Tag requires less than a day at the bench from live cells to sequencing-ready barcoded libraries. Because of low background levels, barcoded and pooled CUT&Tag libraries can be sequenced for ~$25 per sample, enabling routine genome-wide profiling of chromatin proteins and modifications that requires no special skills or equipment.
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