Project description:Sulfate reducing bacteria (SRB), which can produce hydrogen sulfide in sewers, can lead to severe odor problems and asset deterioration due to the sulfide induced concrete corrion. Recently, free nitrou acid (FNA) has been discovered as a promising antimicrobial agent to inhibit SRB activities thereby limiting sulfide production in sewers. However, knowledge of bacteria response dynamics to the increasing levels of FNA is largely unkown.In this study, we detected the whole cell protein production and protein expression dynamics of D. vulgaris by SWATH-MS in the presence of four FNA concentrations (0, 1.0, 4.0 and 8.0 μg N/L) with three treatment time peirods after FNA addition (2, 8 and 12 h). From this a more comprehensive understanding of the FNA effects on D. vulgaris was obtained and the key determinants for withstanding FNA over incubation periods were verified in this sewer corrosion relevant strain.