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Sequoia sempervirens isolate:PB-2020

ABSTRACT: Sequoia sempervirens isolate:PB-2020 Genome sequencing and assembly

PROVIDER: PRJNA606797 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11211700_subreads.fastq.gz	Fastqsanger.gz
	SRR11211701_subreads.fastq.gz	Fastqsanger.gz
	SRR11211702_subreads.fastq.gz	Fastqsanger.gz
	SRR11211703_subreads.fastq.gz	Fastqsanger.gz
	SRR11211704_subreads.fastq.gz	Fastqsanger.gz

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Pb stress and ectomycorrhizas: stronger and more effective proteomic responses in poplar roots inoculated with Paxillus involutus isolate characterised by a low root colonization ratio

Project description:The commonly observed increased heavy metal tolerance of ectomycorrhized plants is usually linked with the protective role of the fungal hyphae covering colonized plant root tips. However, the molecular effects of ECM-triggered increases in plant mass and the ‘dilution effect’ are unknown. Here, we examined Populus × canescens microcuttings inoculated with the Paxillus involutus isolate, which triggered an increase in poplar growth despite successful colonization of only 1.9% ± 0.8 of root tips. Analyzed plants grown for 6 weeks in agar medium enriched with 0.75 mM Pb(NO3)2 lacked a protective fungal biofilter. In minimally colonized ‘bare’ roots, the proteome response to Pb was similar to that in noninoculated plants (e.g., higher abundances of PM- and V-type H+ 31 ATPases and lower abundance of ribosomal proteins). However, the more intensive activation of molecular processes leading to Pb sequestration or redirection of the root metabolic flux into amino acid and Pb chelate (phenolic and citrate) biosynthesis coexisted with lower Pb uptake compared to that in controls. The molecular Pb response of inoculated roots was more intense and effective than that of noninoculated roots in poplars.

2021-09-09 | PXD020049 | Pride

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