Project description:RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. PARE libraries were constructed for both barley organs, followed by sequencing of NGS libraries.
Project description:RNA was isolated from 23 old barley plants (shoots), line Rolap. Libraries were prepared using mRNA-Seq Library kit v2 (Lexogen), followed by sequencing of NGS libraries
Project description:RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. We used a modified method that allows for enrichment of small RNAs. Libraries were prepared using TruSeq Small RNA Library Preparation Kit (Illumina), followed by sequencing of NGS libraries.
Project description:Fourteen days plants growth under hydroponic +P condition (200 µM) were treated with +P(200µM) or –P (no phosphate) for another 7 days, shoot of plants from 3 biological repeats were sampled for Affymetrix microarray analysis. We used microarrays to detail the global programme of gene expression underlying +Pi and -Pi condition between WT and spx1spx2 double mutant.