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The use of autologous peritoneum for mucosal replacement following ileal neobladder construction: a novel technique in a porcine model

ABSTRACT: The use of autologous peritoneum for mucosal replacement following ileal neobladder construction: a novel technique in a porcine model

PROVIDER: PRJNA608128 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11147377_1.fastq.gz	Fastqsanger.gz
	SRR11147377_2.fastq.gz	Fastqsanger.gz
	SRR11147378_1.fastq.gz	Fastqsanger.gz
	SRR11147378_2.fastq.gz	Fastqsanger.gz
	SRR11147379_1.fastq.gz	Fastqsanger.gz

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The use of autologous peritoneum for mucosal replacement following ileal neobladder construction: a novel technique in a porcine model

Project description:Background:The transposed intestinal segments maintain their absorptive and secretive functions which may lead to metabolic disorders after ileal neobladder construction. The aim of this study was to develop a novel technique to reduce the complications of orthotopic urinary diversion in a porcine model. Methods: An ileal neobladder procedure was performed in eight female pigs. Four pigs (experimental group) received mucosal replacement of the neobladder using autologous peritoneum, while the other four pigs (control group) received a normal U-shaped ileal neobladder. After three months, the neobladder was evaluated using IVU, cystograms and cystoscopy. Blood biochemistries, urine samples and venous gas analyses were obtained preoperatively and postoperatively. The neobladder was then harvested to assess pathological findings. Results: The mean (range) operating time was 248 (224-287) min in the control group and 325 (296-360) min in the experimental group. All animals but one from the experimental group survived to the designated endpoints. Postoperative IVU revealed that the urinary tracts were patent and that there was no hydronephrosis or dilated ureter. Cystograms demonstrated a spherical neobladder. Cystoscopy showed a smooth neobladder wall. Histological analysis revealed that the peritoneum-ileal seromuscular flap surface was lined with the urothelial layers. Transcriptome comparison showed the maximum similarity between mucosa of neobladder from experimental group and mucosa of normal bladder. Conclusions: The novel technique is shown to be feasible and reproducible in a porcine model. The imageological, histological, and transcriptometric analyses all confirmed that autologous peritoneum can be used as a natural biological reconstruction material.

2021-04-07 | GSE145751 | GEO

Automating iPSC generation to enable autologous photoreceptor cell replacement therapy

Project description:Automating iPSC generation to enable autologous photoreceptor cell replacement therapy

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Transcriptome analysis of differential gene expression of endometrium, peritoneum, and endometriosis lesions

Project description:Analysis of endometriosis disease associated tissues, endometrium and peritoneum at gene expression level. In this study we present, an interactive web-based user interface for browsing gene expression patterns in the normal endometrium, peritoneum and endometriotic lesions. This tool allows users to explore gene expression profiles of genes of interests in the endometrium and different lesion types without the requirement of advanced computational skills.

2020-07-27 | GSE141549 | GEO

Construction of an artificial diploid Escherichia coli using a CRISPR-based chromosome-doubling technique

Project description:we designed a CRISPR-based chromosome-doubling technique to construct an artificial diploid Escherichia coli cell. The stable diploid E. coli was confirmed by quantitative PCR and third-generation genome sequencing.

2022-12-29 | GSE192799 | GEO

Expression date from mouse peritoneum after intraperitoneal injection of chlorhexidine gluconate

Project description:To study angiogenesis and inflammatory mediators in peritoneal fibrosis, we used microarray analysis and compared gene expression profiles of the peritoneum in control and chlorhexidine gluconate (CG)-induced peritoneal fibrosis mice.

2020-11-24 | GSE162029 | GEO

In vitro effects of equine autologous conditioned serum and autologous equine serum on inflamed chondrocyte pellets

Project description:The aim of the study was to investigate the effects of autologous equine serum (AES) incubated for 24 h and autologous conditioned serum (ACS) on inflamed equine chondrocyte pellets in vitro.

2023-04-19 | GSE152253 | GEO

Sporulation: TAO3 replacement strains

Project description:Sporulation: TAO3 replacement strains

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The Application of a New Framework Construction Technique in Autologous Costal Cartilage Rhinoplasty.

Project description:BackgroundIn this study, the clinical efficacy of a new framework construction technique for costal cartilage rhinoplasty was investigated.MethodsFrom January 2020 to February 2022, patients who underwent rhinoplasty in the department of plastic and reconstructive surgery of Zhejiang Provincial People's Hospital were enrolled. The sixth costal cartilage was made into the nasal columellar support graft (strut), the nasal tip graft integrated scaffold, and the septal extension grafts. The strut and septal extension grafts were sutured to construct the framework. Adobe Photoshop 6.0 was used to measure a series of aesthetic indices preoperatively and at 9 months postoperatively, including the nasal length, tip projection, nasofrontal angle, columellar/lobular angle, and nasolabial angle. The visual analog scale score and rhinoplasty outcome evaluation score were used to assess patient satisfaction. A paired t test was used for data analysis, and a P value of less than 0.05 was considered to indicate statistical significance.ResultsA total of 65 patients aged 19-43 years (average age 26.0 y) were enrolled. No short-term complications occurred. All patients were followed up for 9-23 months. There were statistically significant differences in all measurements (P < 0.05), which suggested that the aesthetic defects of the nose were corrected and that no obvious deflection or rotation of the nasal tip occurred. The visual analog scale score and rhinoplasty outcome evaluation score indicated a significant improvement in patient satisfaction (P < 0.05).ConclusionsThis new framework construction technique for costal cartilage rhinoplasty can reduce the risk of framework deflection and nasal tip rotation and provide a satisfactory nasal columellar shape.

| S-EPMC11658749 | biostudies-literature

RNA-seq comparison: before/after MARIS protocol and library construction technique test

Project description:RNA-seq comparison: before/after MARIS protocol and library construction technique test

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Aberrant expression of the DCT gene is implicated in the hyperpigmentation of the visceral peritoneum of the chicken

Project description:Hyperpigmentation of the visceral peritoneum (HVP) has recently garnered significant attention within the poultry industry due to its potential implications for the health of afflicted animals and its adverse effects on the appearance of commercial chicken carcasses. Unfortunately, the heritable characteristics of HVP are not yet fully understood. Motivated by poultry industry demand, we deployed multi-omics comparing the clinical HVP and normal samples profiling the potential regulating molecular landscape in cell and genome resolution, specifically, aiming to decipher the precise molecular genomic marker. Specifically, we identified the signature genes for the HVP phenotype and found that the signature gene of melanogenesis strongly enriched in the HVP group. At the melanocyte level, overexpression of the DCT gene can promote the proliferation of melanocytes, with exogenous Tyr addition. On the contrary, significantly lower melanin content in the supernatant of cells was detected after siRNA interference relative to untreated cells. Through single-cell sequencing, the hyperexpression of melanogenesis-associated genes in melanocytes was echoed. In summary, molecular features of the HVP phenotype were systematically revealed, which may partially explain the strong heterogeneities of the peritoneum phenotype. The hyperpigmentation of the peritoneum is attributed to an excess deposition of melanin, where the DCT hyperexpression can be regarded as a molecular marker related to the deposition of peritoneal melanin. Future directions should deploy gene editing technology to alert the gene expression of DCT to change the phenotype of pigmentation in the visceral peritoneum.

2024-01-01 | GSE246996 | GEO

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