Ontology highlight
ABSTRACT:
PROVIDER: PRJNA609457 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR11292300_1.fastq.gz | Fastqsanger.gz | |||
| SRR11292300_2.fastq.gz | Fastqsanger.gz | |||
| SRR11292301_1.fastq.gz | Fastqsanger.gz | |||
| SRR11292301_2.fastq.gz | Fastqsanger.gz | |||
| SRR11292302_1.fastq.gz | Fastqsanger.gz |
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Project description:The core Polycomb Repressor Complex 2 (PRC2) is composed of Ezh1/2, Suz12, Eed and is responsible for mediating both H3K27me2 and H3K27me3. However, the mechanisms by which PRC2 demarcates these two repressive modifications in the genome are unknown. In a functional screen, we identified the H3K36 dimethyltransferase Nsd1 as a modulator of PRC2-mediated di- and trimethylation of H3K27. ChIP-Seq analysis following the depletion of Nsd1 revealed a global reduction in H3K36me2 and an increase in H3K27me3 at sites previously marked by H3K27me2. We show that the H3K36me2 at H3K27me2 marks co-occupy regions and provide evidence that the presence of H3K36me2 functions to restrict the spatial distribution of Polycomb mediated H3K27me3 domains.
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| PRJDB4967 | ENA