Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [miRA-seq]

ABSTRACT: Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [miRA-seq]

PROVIDER: PRJNA609696 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11214400.fastq.gz	Fastqsanger.gz
	SRR11214401.fastq.gz	Fastqsanger.gz
	SRR11214402.fastq.gz	Fastqsanger.gz
	SRR11214387.fastq.gz	Fastqsanger.gz
	SRR11214388.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [miRA-seq]

Project description:Zebrafish larvae treated with triptolide (5 dpf larvae after exposure to triptolide (1.6μM) for 6 hours) or vehicle control.

2020-03-03 | GSE146200 | GEO

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model

Project description:Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model

| PRJNA609691 | ENA

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [RNA-seq]

Project description:Zebrafish larvae treated with triptolide (5 dpf larvae after exposure to triptolide (1.6μM) for 6 hours) or vehicle control.

2020-03-03 | GSE146199 | GEO

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [RNA-seq]

Project description:Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [RNA-seq]

| PRJNA609697 | ENA

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model.

Project description:Triptolide is a vine extract used in traditional Chinese medicines and associated with hepatotoxicity. In vitro data suggest that inhibition of RNA synthesis may be the mechanism of toxicity. For studying drug-induced liver injury the zebrafish has experimental, practical and financial advantages compared with rodents. The aim of this study was to explore the mechanism of triptolide toxicity using zebrafish as the model system. The effect of triptolide exposure on zebrafish larvae was determined with regard to mortality, histology, expression of liver specific microRNA-122 and liver volume. Fluorescent microscopy was used to track toxicity in the Tg(-2.8lfabp:GFP)as3 zebrafish line. Informed by microscopy, RNA-sequencing was used to explore the mechanism of toxicity. Triptolide exposure resulted in dose-dependent mortality, a reduction in the number of copies of microRNA-122 per larva, hepatocyte vacuolation, disarray and oncotic necrosis, and a reduction in liver volume. These findings were consistent across replicate experiments. Time-lapse imaging indicated the onset of injury was 6 h after the start of exposure, at which point, RNA-sequencing revealed that 88% of genes were down-regulated. Immune response associated genes were up-regulated in the triptolide-treated larvae including nitric oxide synthase. Inhibition of nitric oxide synthase increased mortality. Triptolide induces hepatotoxicity in zebrafish larvae. This represents a new model of drug-induced liver injury that complements rodents. RNA sequencing, guided by time-lapse microscopy, revealed early down-regulation of genes consistent with previous invitro studies, and facilitated the discovery of mechanistic inflammatory pathways.

| S-EPMC5837554 | biostudies-literature

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-03-03 | GSE146201 | GEO

COHCAP MIRA / 450k Comparison [MIRA]

Project description:Compare a single sample run with two different technologies: Illumina 450k methylation array and MIRA on NimbleGen

2013-06-08 | GSE42734 | GEO

Prunus mira

Project description:Prunus mira overview

| PRJNA594447 | ENA

Scolopax mira

Project description:Scolopax mira overview