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Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [RNA-seq]

ABSTRACT: Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [RNA-seq]

PROVIDER: PRJNA609697 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11214371_1.fastq.gz	Fastqsanger.gz
	SRR11214371_2.fastq.gz	Fastqsanger.gz
	SRR11214372_1.fastq.gz	Fastqsanger.gz
	SRR11214372_2.fastq.gz	Fastqsanger.gz
	SRR11214373_1.fastq.gz	Fastqsanger.gz

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Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model

Project description:Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model

| PRJNA609691 | ENA

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [miRA-seq]

Project description:Zebrafish larvae treated with triptolide (5 dpf larvae after exposure to triptolide (1.6μM) for 6 hours) or vehicle control.

2020-03-03 | GSE146200 | GEO

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [RNA-seq]

Project description:Zebrafish larvae treated with triptolide (5 dpf larvae after exposure to triptolide (1.6μM) for 6 hours) or vehicle control.

2020-03-03 | GSE146199 | GEO

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [miRA-seq]

Project description:Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [miRA-seq]

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Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model.

Project description:Triptolide is a vine extract used in traditional Chinese medicines and associated with hepatotoxicity. In vitro data suggest that inhibition of RNA synthesis may be the mechanism of toxicity. For studying drug-induced liver injury the zebrafish has experimental, practical and financial advantages compared with rodents. The aim of this study was to explore the mechanism of triptolide toxicity using zebrafish as the model system. The effect of triptolide exposure on zebrafish larvae was determined with regard to mortality, histology, expression of liver specific microRNA-122 and liver volume. Fluorescent microscopy was used to track toxicity in the Tg(-2.8lfabp:GFP)as3 zebrafish line. Informed by microscopy, RNA-sequencing was used to explore the mechanism of toxicity. Triptolide exposure resulted in dose-dependent mortality, a reduction in the number of copies of microRNA-122 per larva, hepatocyte vacuolation, disarray and oncotic necrosis, and a reduction in liver volume. These findings were consistent across replicate experiments. Time-lapse imaging indicated the onset of injury was 6 h after the start of exposure, at which point, RNA-sequencing revealed that 88% of genes were down-regulated. Immune response associated genes were up-regulated in the triptolide-treated larvae including nitric oxide synthase. Inhibition of nitric oxide synthase increased mortality. Triptolide induces hepatotoxicity in zebrafish larvae. This represents a new model of drug-induced liver injury that complements rodents. RNA sequencing, guided by time-lapse microscopy, revealed early down-regulation of genes consistent with previous invitro studies, and facilitated the discovery of mechanistic inflammatory pathways.

| S-EPMC5837554 | biostudies-literature

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-03-03 | GSE146201 | GEO

Characterization of Zearalenone-Induced Hepatotoxicity and its Mechanisms by Transcriptomics in Zebrafish Model

Project description:Zearalenone is a mycotoxin produced by several species of Fusarium fungi, which contaminates crop and cereal products worldwide. It mainly has estrogenic, immunotoxic and xenogenic effects on various organisms. Though its hepatotoxicity has been reported by few studies, the underlying mechanisms are yet to be investigated. This study aimed to comprehensively evaluate the hepatotoxic effects of zearalenone and its molecular mechanism in the zebrafish model system.

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Mercury-Induced Hepatotoxicity in Zebrafish

Project description:Full title: Mercury-Induced Hepatotoxicity in Zebrafish: In Vivo Mechanistic Insights from Transcriptome Analysis, Phenotype Anchoring and Targeted Gene Expression Validation In this study, we performed microarray-based expression profiling on liver of zebrafish exposed to 200 Âµg/L of mercuric chloride for 8-96 h, to identify global transcriptional programs and biological pathways involved in mercury-induced adaptive responses under in vivo environment. We analyzed 12 arrays for mercuric chloride treated zebrafish liver and 12 arrays for control liver.

2010-06-05 | E-GEOD-18861 | biostudies-arrayexpress

Mercury-Induced Hepatotoxicity in Zebrafish

Project description:Full title: Mercury-Induced Hepatotoxicity in Zebrafish: In Vivo Mechanistic Insights from Transcriptome Analysis, Phenotype Anchoring and Targeted Gene Expression Validation In this study, we performed microarray-based expression profiling on liver of zebrafish exposed to 200 µg/L of mercuric chloride for 8-96 h, to identify global transcriptional programs and biological pathways involved in mercury-induced adaptive responses under in vivo environment.

2010-03-31 | GSE18861 | GEO

Characterization of Zearalenone-Induced Hepatotoxicity and its Mechanisms by Transcriptomics in Zebrafish Model

Project description:Characterization of Zearalenone-Induced Hepatotoxicity and its Mechanisms by Transcriptomics in Zebrafish Model

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