Project description:We carried out genomewide DNA methylation analysis by whole genome bisulfite sequencing to identify candidate genes involved in pear fruit semi-russet formation, by comparing the CK (russet) and bagging treated (green) ‘Cuiguan’ pear fruit skin 115 DAFB.
Project description:Using whole genome bisulfite sequencing to provide single-base resulution of DNA methylation status in pear fruits ( "Suli", Pyrus bretschneideri Rdhd. ) in four different stages.
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Project description:purpose：The purpose of this study was to compare the transcriptome data of the diseased and the non diseased pear to verify the changes of different physiological indexes of the diseased pear Methods：By sequencing the transcriptome of the treatment group and the control group, the differences of genes related to glycoalcohol metabolism and cell wall physiological pathway metabolism between the two groups were compared. Verification by fluorescence quantitative PCR
Project description:Comparative analyze at the transcriptomic level 1) of Venturia pyrina pear host resistance via the major apple resistance gene Rvi6, in Rvi6 overexpressing transgenic pear versus ‘conference’ susceptible variety; 2) of Venturia inaequalis pear nonhost resistance, in ‘Conference’ variety, 24 and 72 hours post inoculation.
