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Analysis of mRNA expression in human skin fibroblasts WS1 cells between IFI6-overexpression and control upon irradiation.

ABSTRACT: Analysis of mRNA expression in human skin fibroblasts WS1 cells between IFI6-overexpression and control upon irradiation.

PROVIDER: PRJNA610807 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11251078_1.fastq.gz	Fastqsanger.gz
	SRR11251078_2.fastq.gz	Fastqsanger.gz
	SRR11251079_1.fastq.gz	Fastqsanger.gz
	SRR11251079_2.fastq.gz	Fastqsanger.gz
	SRR11251080_1.fastq.gz	Fastqsanger.gz

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Analysis of mRNA expression in human skin fibroblasts WS1 cells between IFI6-overexpression and control upon irradiation.

Project description:This study aims to compared mRNA expression between IFI6-overexpressed WS1 cells and NC by RNA-Seq. In this study, WS1 cells were divided into two groups and each group had four repeats, one group was infected by IFI6-overexpressed lentivirus and another was infected by NC lentivirus. The two groups of cells were then irradiated with a single 5 Gy dose of radiation at a fixed rate of 1.15 Gy/min using an X-ray linear accelerator (Rad Source Technologies Inc., Suwanee, GA, USA). 24 hours post irradiation, WS1 cells from IFI6-overexpression and NC were collected and subjected to mRNA expression analysis.

2020-09-09 | GSE146527 | GEO

lncRNA and mRNA-sequencing of WS1 cells after 5Gy of X-Ray irradiation

Project description:Purpose: The goal of this study was to compare the lncRNA and mRNA expression profile of human fibroblast WS1 cells between 0 Gy and 5 Gy X-ray irradiation. Methods: lncRNA and mRNA-sequencing of WS1 cells were generated using Illuminal Hiseq4000, RNA-seq reads were mapped to human genome hg19 by hisat2 software. Cuffdiff software was used to analysis the differentially expressed levels of LncRNA and mRNA FPKM. Results: We identified different expressed levels of 88780 lnRNA and 20311 mRNA transcripts in WS1 cells between 0 Gy and 5 Gy X-ay irradiation. Conclusion: Our study first revealed the detail of lncRNA and mRNA expression profiles of WS1 cells after 5Gy of X-Ray irradiation

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Transcriptional profile of human STAT1-/- fibroblasts expressing IFI6 or empty control vector

Project description:Detection of viral infection by pattern-recognition receptors triggers production of interferon. Secreted interferon binds to cognate receptors, triggering JAK/STAT signaling, resulting in the transcription and production of hundreds of interferon-stimulated genes (ISGs). Our lab identified interferon alpha inducible protein 6 (IFI6) as an ISG that potently suppresses replication of viruses from the Flavivirus genus. To test whether the inhibitory effects of IFI6 were due to activating expression of other antiviral ISGs, we overexpressed IFI6 and a control vector and examined global transcription using RNA-Seq.

2018-12-01 | GSE105771 | GEO

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Project description:We report the application of RIP-sequencing technology for high-throughput profiling of ELAVL1 protein on the effect of lncRNA an mRNA in WS1 cells after 5 Gy X-ray irradiation

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Project description:High throughput sequencing of human fibroblast WS1 cells after 5 Gy X-ray irradiation

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Pan-cancer analysis of IFI6 and its tumor promotor effects in esophageal squamous cell carcinoma

Project description:IFI6 is one of the ISG family, which is considered to play an important role in signal transduction pathway and immune response. However, there are few reports on IFI6, and only a few studies have reported that IFI6 may play a role in the occurrence and development of some tumors. We downloaded normal tissue and tumor tissue information from GTEx, TCGA and CCLE, and used survival analysis to study the relationship between IFI6 expression and OS, DSS, etc. We found that IFI6 was expressed in a variety of tumors, and the expression of individual tumors such as LUAD was very high. In addition, we also studied the relationship between IFI6 and immune cell infiltration enrichment, tumor immune microenvironment, methylation and so on. Furthermore, the high expression level of IFI6 was verified in esophageal squamous cell carcinoma (ESCC). In ESCC cells, IFI6 overexpression significantly promoted cell proliferation and growth, and inhibited cell apoptosis. In the lncRNA sequencing analysis, we found that IFI6 could regulate the expression of multiple genes, including HNRNPC, PEMT, INTS7, and several pathways, such as VEGF signaling pathway, and PPAR signaling pathway. Our study provides new insights into the role of IFI6 in pan-cancer. IFI6 may represent a novel target for the treatment of esophageal squamous cell carcinoma.

2021-04-29 | GSE173475 | GEO

the effect of UIFSP on gene expression of WS1 cells after UVB exposure

Project description:to investigate the function mechanism of UIFSP pretreatment in WS1 cells exposure to UVB,and how UIFSP regulate cell gene expression and signal transduction.

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